Serpins from an insect, Manduca sexta.
Serpins from an insect, Manduca sexta.
复制标题
来自昆虫 Manduca sexta 的丝氨酸蛋白酶抑制剂。
DOI：
10.1007/978-1-4615-5391-5_15
复制
发表时间：
1997
影响因子：
--
通讯作者：
Jiang,H
中科院分区：
文献类型：
--
作者：
Kanost,MR;Jiang,H
丝氨酸蛋白酶抑制剂超家族的成员已在几种无脊椎动物中被鉴定，主要是节肢动物门动物血淋巴中的蛋白质9。丝氨酸蛋白酶抑制剂cDNA已从马蹄蟹14、15、小龙虾13、两种鳞翅目昆虫6、10、17、20、24、果蝇黑腹果蝇3、血吸虫属吸虫2和寄生线虫25中克隆。在马蹄蟹和昆虫的血淋巴中存在具有不同蛋白酶抑制活性的丝氨酸蛋白酶抑制剂，这表明丝氨酸蛋白酶抑制剂可能在无脊椎动物的几个生理过程中发挥作用，就像它们在哺乳动物中一样。无脊椎动物物种的数量远远超过脊椎动物的数量，生物多样性更大。因此，可以预见，随着对无脊椎动物生物化学研究的深入，将会发现大量具有不同活性和功能的丝氨酸蛋白酶抑制剂。在我们对烟草天蛾（Manduca sexta）丝氨酸蛋白酶抑制剂的研究中，我们已经确定了一个具有不同抑制活性的丝氨酸蛋白酶抑制剂家族，以及产生反应位点多样性的独特丝氨酸蛋白酶抑制剂基因结构。
Members of the serpin superfamily have been identified in several invertebrate species, primarily as proteins in the hemolymph of animals from the phylum Arthropoda9. Serpin cDNAs have been cloned from horseshoe crabs14,15, a crayfish13, two species of lepidopteran insects6,10,17,20,24, the fruit flyDrosophila melanogaster3, trematodes from the genusSchistosoma2, and from a parasitic nematode25. The presence of serpins with differing proteinase inhibitory activities in the hemolymph of horseshoe crabs and insects suggests that serpins may function in several physiological processes in invertebrates, as they do in mammals. The number of invertebrate species vastly exceeds the number of vertebrates, with far greater biological diversity. Thus, it may be predicted that as biochemical studies on invertebrates progress, a large number of serpins with diverse activities and functions will be discovered. In our research on serpins from the tobacco hornworm,Manduca sexta, we have identified a family of serpins with different inhibitory activities, and a unique serpin gene structure for generating reactive site diversity.