Expression of tumor necrosis factor-α in regenerating muscle fibers in inflammatory and non-inflammatory myopathies
Expression of tumor necrosis factor-α in regenerating muscle fibers in inflammatory and non-inflammatory myopathies
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DOI：
10.1007/s00401-002-0632-4
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发表时间：
2003-03-01
影响因子：
12.7
通讯作者：
Sobue, G
中科院分区：
文献类型：
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作者：
Kuru, S;Inukai, A;Sobue, G
肿瘤坏死因子-α在特发性炎症性肌病和Duchenne肌营养不良症(DMD)中的表达水平升高，但其确切作用尚不清楚。为了阐明肿瘤坏死因子-α在多发性肌炎(PM)、皮肌炎(I)M和DMD中的表达，我们用原位杂交和免疫组织化学方法研究了肿瘤坏死因子-α及其受体在多发性肌炎(PM)、皮肌炎(I)M和DMD中的表达。我们发现，在肌肉纤维中存在肿瘤坏死因子-α的mRNA和蛋白。在PM、DM和DMD中均可观察到肿瘤坏死因子-α阳性纤维，但在神经源性疾病和正常对照组中则很少或不表达。肿瘤坏死因子-α阳性纤维比例与肌球蛋白重链发育形式(MHC-d)阳性再生纤维比例呈显著正相关。肿瘤坏死因子受体阳性纤维数量较少。部分肌纤维同时表达肿瘤坏死因子-α及其受体。我们的结果表明，TNF-α是由肌肉纤维产生和表达的，并与肌肉再生有关。
The expression level of tumor necrosis factor (TNF)-alpha is elevated in idiopathic inflammatory myopathies and Duchenne muscular dystrophy (DMD), but the precise role of TNF-alpha is unknown. To elucidate the possible role of TNF-alpha, we investigated the expression of TNF-alpha and its receptor in polymyositis (PM), dermatomyositis (I)M), and DMD using in situ hybridization (ISH) and immunohistochemistry. We showed that TNF-alpha mRNA and protein were present in muscle fibers. TNF-alpha-positive fibers were observed in all cases of PM, DM and DMD, but were rare or absent in neurogenic disorders and normal controls. The proportion of TNF-alpha-positive fiber showed a significant positive correlation with the proportion of regenerating fibers that were positive for the developmental form of myosin heavy chain (MHC-d). The number of TNF receptor-positive fibers was small. Some muscle fibers expressed both TNF-alpha and its receptor simultaneously. Our results indicate that TNF-alpha is produced and expressed by muscle fibers and associated with muscle regeneration.