Structure of fucose branches in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus
Structure of fucose branches in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus
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DOI：
10.1016/s0008-6215(96)00258-3
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发表时间：
1997-01-17
影响因子：
3.1
通讯作者：
Ishii, T
中科院分区：
文献类型：
--
作者：
Kariya, Y;Watabe, S;Ishii, T
从海参（Stichopus Stichopus）体壁中制备了岩藻糖支化硫酸软骨素E。将纯化的糖胺聚糖（GAG）化学酯化，然后进行羧基还原。对完整的、酯化的和酯化的/羧基还原的GAG组分进行全O-甲基化。所得部分甲基化的糖醇乙酸酯的GC-MS分析表明，岩藻糖分支由通过位置（1 -> 3）糖苷连接的两个岩藻吡喃糖残基形成，并且岩藻糖分支和葡萄糖醛酸几乎是等摩尔的。此外，阐明了约20%的分支从核心硫酸软骨素聚合物的葡萄糖醛酸部分的O-3位延伸，而假定剩余的岩藻糖分支从N-乙酰半乳糖胺部分的O-4和/或O-6位突出。根据甲基化分析中的六种取代模式，该岩藻糖分支也被证实高度硫酸化。[图片]（C）1997 Elsevier Science Ltd.
Fucose-branched chondroitin sulfate E was prepared from the body wall of sea cucumber Stichopus japonicus. The purified glycosaminoglycan (GAG) was chemically desulfated, followed by carboxyl reduction. Intact, desulfated, and desulfated/carboxyl-reduced GAG fractions were subjected to per-O-methylation. GC-MS analyses of the resultant partially methylated alditol acetates demonstrated that the fucose branch is formed by two fucopyranosyl residues linked glycosidically through position (1 --> 3), and that the fucose branch and glucuronic acid are almost equimolar. In addition, it was elucidated that about 20% of the branches stretch from O-3 position of a glucuronic acid moiety of the core chondroitin sulfate polymer, while remaining fucose branches are postulated to protrude from O-4 and/or O-6 position(s) of a N-acetylgalactosamine moiety. This fucose branch was also confirmed to be highly sulfated according to six kinds of substitution pattern in methylation analysis.[GRAPHICS](C) 1997 Elsevier Science Ltd.