Differences in the frequency of cytokine-producing cells in antigenemic and nonantigenemic individuals with bancroftian filariasis
Differences in the frequency of cytokine-producing cells in antigenemic and nonantigenemic individuals with bancroftian filariasis
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DOI：
10.1128/iai.66.4.1377-1383.1998
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发表时间：
1998-04-01
影响因子：
3.1
通讯作者：
Freedman, DO
中科院分区：
文献类型：
--
作者：
de Almeida, AB;Silva, MCME;Freedman, DO
具有淋巴丝虫病临床表现的个体可能目前已感染或未感染。来自巴西班氏吴策线虫流行区的25个个体被分类为无症状微丝蚴血症个体、具有临床丝虫病的抗原血症个体或具有临床丝虫病的非抗原血症个体，有丝分裂原刺激的外周血单个核细胞（PBMC）的细胞内细胞因子染色显示，γ干扰素的频率临床丝虫病非抗原血症个体产生IFN-γ或白细胞介素-4（IL-4）的细胞高于无症状微丝蚴血症个体（几何平均值，分别为22.1与10.7%（P = 0.02）和2.9与1.4%（P = 0.01））。当无症状的微丝蚴血症个体和临床丝虫病的抗原血症个体被分组在一起构成所有活动性感染个体时，IFN-γ产生细胞的频率也低于临床丝虫病的非抗原血症个体（P = 0.04）。同样，在活跃感染的个体中产生IL-4的细胞的频率也低于临床丝虫病的非抗原血症个体（P = 0.02）。各组之间纯化的CD 4 T淋巴细胞中产生IFN-γ、IL-4或IL-5的细胞频率没有差异。这些研究结果表明，抗原血症的存在，这是目前的活动性感染的指标，是密切相关的频率IFN-γ和IL-4产生细胞的淋巴丝虫病。在三组中发现的产生精氨酸的细胞频率的差异似乎是由于CD 4 T细胞以外的细胞亚群。
Individuals with clinical manifestations of lymphatic filariasis may be currently infected or not. Twenty-five individuals from a Wuchereria bancrofti-endemic area of Brazil,were classified as being asymptomatic microfilaremic individuals, antigenemic individuals with clinical filariasis, or nonantigenemic individuals with clinical filariasis, Intracellular cytokine staining of mitogen-stimulated peripheral blood mononuclear cells (PBMC) showed that the frequency of either gamma interferon (IFN-gamma)- or interleukin-4 (IL-4)-producing cells was higher in the nonantigenemic individuals with clinical filariasis than in the asymptomatic microfilaremic individuals (geometric means, 22.1 versus 10.7% (P = 0.02) and 2.9 versus 1.4% (P = 0.01), respectively). When the asymptomatic microfilaremic individuals and antigenemic individuals with clinical filariasis were grouped together to constitute all actively infected individuals, the frequency of IFN-gamma-producing cells was also lower than in the nonantigenemic individuals with clinical filariasis (P = 0.04). Likewise, the frequency of IL-4-producing cells in the actively infected individuals was also lower than in the nonantigenemic individuals with clinical filariasis (P = 0.02). No differences in the frequency of IFN-gamma-, IL-4-, or IL-5-producing cells in purified CD4 T lymphocytes were found among the groups. These findings suggest that the presence of antigenemia, which is an indicator of current active infection, is closely associated with the frequency of IFN-gamma- and IL-4-producing cells in lymphatic filariasis. The differences found in the frequency of cytokine-producing cells among the three groups appear to be due to a subset of cells other than CD4 T cells.