Identification of porcine circovirus type 2 in retrospective cases of pigs naturally infected with porcine epidemic diarrhoea virus
Identification of porcine circovirus type 2 in retrospective cases of pigs naturally infected with porcine epidemic diarrhoea virus
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DOI：
10.1016/j.tvjl.2004.09.002
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Chae, C
中科院分区：
文献类型：
--
作者：
Jung, K;Ha, Y;Chae, C
用聚合酶链式反应(PCR)和原位杂交法对新鲜肠道组织和福尔马林固定、石蜡包埋的肠道组织中猪圆环病毒2型(PCV2)的鉴定进行了研究。这些组织来自自然感染猪流行性腹泻病毒(PEDV)的猪。在107份自然感染PEDV的猪小肠标本中，共检出35份阳性，阳性率为32.7%。用原位杂交法检测107份自然感染PEDV猪的小肠标本中有32份(29.9%)检出PCV2阳性信号。空肠和回肠的阳性细胞呈多灶性或斑片状分布。在小肠的固有层中发现了明显的阳性标记。本研究的结果表明，PCV2在自然感染PEDV的猪中高度流行。(C)2004爱思唯尔有限公司。保留所有权利。
The identification of porcine circovirus type 2 (PCV2) was studied in fresh intestinal tissues by polymerase chain reaction (PCR) and in formalin-fixed, paraffin-wax-embedded intestinal tissues by in situ hybridisation. The tissues came from pigs naturally infected with porcine epidemic diarrhoea virus (PEDV). A total of 35 (32.7%) of 107 small intestinal samples from pigs naturally infected with PEDV were found to be positive using PCR. Positive signals for PCV2 were detected in 32 (29.9%) of 107 small intestinal samples from pigs naturally infected with PEDV by in situ hybridisation. The distribution of positive cells in the jejunum and ileum was multifocal or patchy. Distinct positive labelling was found throughout the lamina propria in the small intestines. The results of this study indicate that PCV2 is highly prevalent in pigs naturally infected with PEDV. (c) 2004 Elsevier Ltd. All rights reserved.