Organization of Embryonic Morphogenesis via Mechanical Information
Organization of Embryonic Morphogenesis via Mechanical Information
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DOI：
10.1016/j.devcel.2019.05.014
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发表时间：
2019-06-17
影响因子：
11.8
通讯作者：
Holley, Scott A.
中科院分区：
文献类型：
--
作者：
Das, Dipjyoti;Julich, Dorthe;Holley, Scott A.
胚胎组织者建立了可扩散信号分子的梯度，以塑造周围细胞的模式。在这里，我们阐明了胚胎组织者的另一种机制，这是形态因子信号传导的次要后果。利用药理学和局部转基因扰动、斑马鱼胚胎的4D成像、细胞运动的系统分析和计算模型，我们发现脊椎动物尾巴组织者在形态发生信号传导范围之外的距离上协调形态发生。在胚胎伸长过程中，组织者利用机械信息调节细胞运动的速率和一致性，这些机械信息通过相邻细胞之间的接力传递。这种机制类似于颗粒介质和其他堵塞系统中的压力锋，但在胚胎中，机械信息来自自我推进的细胞运动，而不是细胞之间的力传递。繁殖可能依赖于局部生化信号，影响细胞收缩性、细胞粘附性和/或细胞极性，但不依赖于转录和翻译。
Embryonic organizers establish gradients of diffusible signaling molecules to pattern the surrounding cells. Here, we elucidate an additional mechanism of embryonic organizers that is a secondary consequence of morphogen signaling. Using pharmacological and localized transgenic perturbations, 4D imaging of the zebrafish embryo, systematic analysis of cell motion, and computational modeling, we find that the vertebrate tail organizer orchestrates morphogenesis over distances beyond the range of morphogen signaling. The organizer regulates the rate and coherence of cell motion in the elongating embryo using mechanical information that is transmitted via relay between neighboring cells. This mechanism is similar to a pressure front in granular media and other jammed systems, but in the embryo the mechanical information emerges from self-propelled cell movement and not force transfer between cells. The propagation likely relies upon local biochemical signaling that affects cell contractility, cell adhesion, and/or cell polarity but is independent of transcription and translation.