Tissue-engineered matrices as functional delivery systems: adsorption and release of bioactive proteins from degradable composite scaffolds.
Tissue-engineered matrices as functional delivery systems: adsorption and release of bioactive proteins from degradable composite scaffolds.
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作为功能传递系统的组织工程基质：从可降解复合支架中吸附和释放生物活性蛋白。
DOI：
10.1002/jbm.a.32722
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Laurencin,CatoT
中科院分区：
文献类型：
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作者：
Cushnie,EmilyK;Khan,YusufM;Laurencin,CatoT
组织工程骨移植物应在形式和功能上模仿理想的自体移植物。然而，具有用于移植应用的适当化学和机械性质的生物材料通常缺乏可增强再生的生物组分。因此，向支架中添加蛋白质（如生长因子）的概念已成为改善整体移植物设计的可能解决方案。在这项研究中，我们研究了这一概念，通过加载多孔羟基磷灰石-聚（丙交酯-共-乙交酯）（HA-PLAGA）支架与模型蛋白，细胞色素c，然后研究其在磷酸盐缓冲盐水溶液中的释放。HA‐PLAGA支架先前已被证明具有生物活性、骨传导性，并具有适合组织工程应用的物理特性。负载实验表明，HA-PLAGA支架也可以有效地作为蛋白质吸附和释放的基质。支架蛋白吸附负载（与基质内的物理截留相反）与支架HA含量水平直接相关。在水性缓冲液中孵育长达8周后，支架的HA相促进蛋白质保留在基质中。更高水平的蛋白质保留时间可以通过增加蛋白质-细胞相互作用的可能性来改善蛋白质的有效活性。通过HA‐PLAGA支架的组成简单地控制蛋白质加载和递送的能力提供了形成稳健的功能化骨移植物的潜力。© 2010 Wiley Periodicals，Inc. J Biomed Mater Res Part A，2010
A tissue‐engineered bone graft should imitate the ideal autograft in both form and function. However, biomaterials that have appropriate chemical and mechanical properties for grafting applications often lack biological components that may enhance regeneration. The concept of adding proteins such as growth factors to scaffolds has therefore emerged as a possible solution to improve overall graft design. In this study, we investigated this concept by loading porous hydroxyapatite‐poly(lactide‐co‐glycolide) (HA‐PLAGA) scaffolds with a model protein, cytochrome c, and then studying its release in a phosphate‐buffered saline solution. The HA‐PLAGA scaffold has previously been shown to be bioactive, osteoconductive, and to have appropriate physical properties for tissue engineering applications. The loading experiments demonstrated that the HA‐PLAGA scaffold could also function effectively as a substrate for protein adsorption and release. Scaffold protein adsorptive loading (as opposed to physical entrapment within the matrix) was directly related to levels of scaffold HA‐content. The HA phase of the scaffold facilitated protein retention in the matrix following incubation in aqueous buffer for periods up to 8 weeks. Greater levels of protein retention time may improve the protein's effective activity by increasing the probability for protein–cell interactions. The ability to control protein loading and delivery simply via composition of the HA‐PLAGA scaffold offers the potential of forming robust functionalized bone grafts. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010