Genome-wide analysis of long non-coding RNAs and their association with wing development in Aphis citricidus (Hemiptera: Aphididae)
Genome-wide analysis of long non-coding RNAs and their association with wing development in Aphis citricidus (Hemiptera: Aphididae)
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长非编码 RNA 的全基因组分析及其与柑橘蚜（半翅目：蚜科）翅膀发育的关系
DOI：
10.1016/j.ibmb.2021.103666
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发表时间：
2021-10-18
影响因子：
3.8
通讯作者：
Wang, Jin-Jun
中科院分区：
文献类型：
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作者：
Shang, Feng;Ding, Bi-Yue;Wang, Jin-Jun
长链非编码RNA（lncRNA）在昆虫的多种生理过程中起着重要作用。翅膀是一种成功的适应，允许昆虫逃离不利的环境，而与翅膀发育相关的lncRNA的信息是有限的。在这项研究中，我们构建了12个文库，从两个RNA-seq比较：4龄有翅蚜虫与有翅成虫和4龄无翅蚜虫与无翅成虫，在棕色柑橘蚜虫Aphis citricidus，以确定翅膀发育相关的lncRNA。共检测到2914个lncRNA，其中50个lncRNA在4龄有翅夜蛾向有翅成虫转变过程中发生了差异表达，28个lncRNA在4龄无翅夜蛾向无翅成虫转变过程中发生了变化。根据组合双翼型中的表达模式，将差异表达的lncRNA分为6个簇。lncRNA Ac_lnc54106.1在4龄有翅蝗向有翅成虫的转变过程中表达上调，但在4龄无翅蝗向无翅成虫的转变过程中表达不明显，这表明lncRNA Ac_lnc54106.1在翅发育的特异性调控中起着重要作用。Ac_lnc54106.1的RNA干扰导致畸形翅。靶标预测、表达模式和RNAi实验结果表明，Ac_lnc54106.1可能靶向PiggyBac转座元件衍生蛋白4（PGBD 4）基因，降低典型翅发育相关基因的表达，最终调控翅发育。蚜虫中lncRNA的系统鉴定增加了我们对非编码RNA如何介导昆虫翅膀可塑性的理解。
Long non-coding RNAs (lncRNAs) play critical roles in the various physiological processes of insects. The wing is a successful adaptation allowing insects to escape from unfavorable environments, while information on lncRNAs related to wing development is limited. In this study, we constructed 12 libraries from two RNA-seq comparisons: 4th instar winged nymphs versus winged adults and 4th instar wingless nymphs versus wingless adults in the brown citrus aphid Aphis citricidus, to identify the wing development-associated lncRNAs. A total of 2914 lncRNAs were identified and 50 lncRNAs were differentially expressed during the 4th instar winged nymphs to winged adults transition, and 28 lncRNAs changed during the 4th instar wingless nymphs to wingless adults transition. The differentially expressed lncRNAs were grouped into six clusters according to the expression patterns in the combined two-winged morphs. lncRNA Ac_lnc54106.1 was up-regulated during 4th instar winged nymphs to winged adults transition, but a lack of change during the 4th instar wingless nymphs to wingless adults transition implied a critical role in the specific regulation of wing development. RNA interference of Ac_lnc54106.1 resulted in malformed wings. Targets prediction, expression patterns, and RNAi assay results showed that Ac_lnc54106.1 may target the PiggyBac transposable element-derived protein 4 (PGBD4) gene, decrease expression of the canonical wing development-related genes, and finally regulate wing development. The systematic identification of lncRNAs in an aphid increases our understanding of how non-coding RNA mediates the wing plasticity of insects.