Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer
Thermal effects on microbial composition and microbiologically induced corrosion and mineral precipitation affecting operation of a geothermal plant in a deep saline aquifer
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DOI：
10.1007/s00792-013-0518-8
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发表时间：
2013-01
期刊：
影响因子：
2.9
通讯作者：
S. Lerm;Anke Westphal;R. Miethling-Graff;M. Alawi;A. Seibt;M. Wolfgramm;H. Würdemann
中科院分区：
文献类型：
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作者：
S. Lerm;Anke Westphal;R. Miethling-Graff;M. Alawi;A. Seibt;M. Wolfgramm;H. Würdemann
对德国北部盆地用于地热储热的深层咸水层的微生物多样性进行了调查。基因指纹分析揭示了含水层冷侧和暖侧产生的流体中不同的微生物群落。通过实时 PCR 对 16S rRNA 基因和异化亚硫酸盐还原酶 (dsrA) 基因进行直接细胞计数和定量，证明了液体中不同的种群大小，与温热液体相比，冷液体中细菌和硫酸盐还原菌 (SRB) 的丰度更高。热井中依赖于操作的温度升高可能提高了有机质的利用率，在流体温度降低后有利于上部设施中发酵细菌和SRB的生长。在冷井中，SRB 占主导地位，并且可能是造成潜井泵腐蚀损坏以及近井眼区域和上部设施过滤器中硫化铁沉淀的原因。这对应于冷井产生的流体中硫酸盐含量较低以及可能因腐蚀释放的氢气含量较高，并且可能有利于氢营养型SRB的生长。这项研究反映了微生物种群对地热发电厂运行的高度影响，因为微生物引起的沉淀和腐蚀过程会对发电厂的可靠性产生不利影响。
The microbial diversity of a deep saline aquifer used for geothermal heat storage in the North German Basin was investigated. Genetic fingerprinting analyses revealed distinct microbial communities in fluids produced from the cold and warm side of the aquifer. Direct cell counting and quantification of 16S rRNA genes and dissimilatory sulfite reductase (dsrA) genes by real-time PCR proved different population sizes in fluids, showing higher abundance ofbacteriaand sulfate reducing bacteria (SRB) in cold fluids compared with warm fluids. The operation-dependent temperature increase at the warm well probably enhanced organic matter availability, favoring the growth of fermentative bacteria and SRB in the topside facility after the reduction of fluid temperature. In the cold well, SRB predominated and probably accounted for corrosion damage to the submersible well pump and iron sulfide precipitates in the near wellbore area and topside facility filters. This corresponded to lower sulfate content in fluids produced from the cold well as well as higher content of hydrogen gas that was probably released from corrosion, and maybe favored growth of hydrogenotrophic SRB. This study reflects the high influence of microbial populations for geothermal plant operation, because microbiologically induced precipitative and corrosive processes adversely affect plant reliability.