The signaling pathway for β1-integrin/ERKs is involved in the adaptation of adipocyte functions to cell size
The signaling pathway for β1-integrin/ERKs is involved in the adaptation of adipocyte functions to cell size
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DOI：
10.1111/j.1749-6632.2002.tb04706.x
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发表时间：
2002-01-01
期刊：
影响因子：
--
通讯作者：
Bazin, R
中科院分区：
文献类型：
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作者：
Farnier, C;Krief, S;Bazin, R
白色脂肪细胞具有显著的储存能力。因此，在渗透冲击后，它们比通常在其他细胞类型中观察到的体积变化幅度更大。肥胖症是脂肪细胞肥大，并且已知代谢功能（脂质的储存和动员以及激素分泌）在脂肪组织的发育过程中会发生变化。1由于肥胖的特征是营养和激素异常，目前尚不清楚肥胖受试者脂肪细胞增大的变化是否仅归因于这些异常。然而，在非肥胖的健康受试者中，脂肪细胞体积目前也增加了20至30倍（直径增加3倍相当于体积增加33倍或27倍）。当在相同的脂肪位置存在非常不同大小的脂肪细胞时，关于脂肪细胞代谢作为细胞大小的函数知之甚少。因此，这项工作的第一个目的是检查相同的脂肪库（在大鼠中），以便我们可以确定脂肪细胞的几种代谢功能，独立于营养或激素条件的变化，是否随细胞大小而变化。从附睾脂肪组织中分离出两个脂肪细胞群（52和22× 103 µ3），我们检测了与脂肪细胞主要功能有关的几种蛋白质的酶活性和表达：（1）储存（葡萄糖转运蛋白Glut 4 mRNA浓度，脂蛋白脂肪酶LPL和脂肪酸合成酶FAS的活性和mRNA浓度）;（2）脂质动员（体外脂肪分解和脂肪敏感脂肪酶（HSL）的表达）;（3）激素产生（瘦素mRNA浓度）。我们的结果（表1）表明，表达式的值-
White adipocytes have significant storage capacity. Accordingly, they are subject to volume changes of greater amplitude than those usually observed in other cell types after osmotic shocks. Obesity worsens fat cell hypertrophy, and it is known that metabolic functions (storage and mobilization of lipids and hormonal secretion) are subject to change during the development of adipose tissue. 1 Since obesity is characterized by nutritional and hormonal abnormalities, it is not known whether the changes observed in enlarged fat cells of obese subjects are solely attributable to these abnormalities. However, in nonobese healthy subjects, adipose cell volume also increases currently by 20 to 30 times (a 3-fold increase in diameter is equivalent to a 33-fold increase, or 27-fold increase, in volume). When adipocytes of very different sizes are present at the same adipose location, little is known about fat cell metabolism as a function of cell size. Thus, the first aim of this work was to examine the same fat depot (in rats) so that we might determine whether several metabolic functions of adipocytes, independent of changes in nutritional or hormonal conditions, were subject to change with cell size. Two populations of adipocytes were isolated from epididymal adipose tissue (52 and 22× 103 µ3), and we examined enzyme activity and expression of several proteins involved in the main functions of adipose cells:(1) storage (glucose transporter Glut4 mRNA concentration, both activities and mRNA concentrations of lipoprotein lipase, LPL, and fatty acid synthase, FAS);(2) mobilization of lipids (in vitro lipolysis and expression of hormone-sensitive lipase, HSL);(3) hormonal production (leptin mRNA concentration). Our results (TABLe 1) show that values for the expres-