Metformin ameliorates insulitis in STZ-induced diabetic mice.
Metformin ameliorates insulitis in STZ-induced diabetic mice.
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作者：
Han X;Tao YL;Deng YP;Yu JW;Cai J;Ren GF;Sun YN;Jiang GJ
二甲双胍是目前应用最广泛的糖尿病一线降糖药。除了降血糖作用外，人们对该药潜在的抗炎作用越来越感兴趣。在本研究中，我们研究了二甲双胍对stz诱导的糖尿病小鼠实验性胰岛素炎的作用。STZ致急性糖尿病小鼠灌胃二甲双胍。测定各组血糖、体重变化及每日摄食量和饮水量。收集胰腺组织进行组织学分析。采用病理评估和免疫组化分析确定二甲双胍对胰岛素炎的影响。ELISA法检测胰腺炎性细胞因子及胰岛素水平。二甲双胍显著降低了实验性糖尿病小鼠的血糖水平，改善了异常的饮水行为。二甲双胍治疗的动物在体重和食物摄入行为方面没有观察到显著差异。在stz诱导的糖尿病模型中，我们发现出现明显的胰岛素炎。然而，二甲双胍给药降低了盲病理评分评估胰岛素炎的严重程度。此外，二甲双胍治疗改善了实验性糖尿病小鼠的胰岛素水平。ELISA检测显示，二甲双胍治疗后胰腺组织中炎症反应标志物IL-1β和TNF-α水平降低。二甲双胍减轻stz诱导的糖尿病小鼠模型的胰岛素炎。这种保护胰岛的作用可能与二甲双胍的抗炎作用部分相关。
Metformin is currently the most widely used first-line hypoglycemic agent for diabetes mellitus. Besides glucose-lowering action, there is increasingly interest in the potential anti-inflammatory action of this drug. In the present study, we investigated the actions of metformin on experimental insulitis using STZ-induced diabetic mice. Mice with acute diabetes induced by STZ were administered metformin by gavage. Changes of blood glucose and body weight, and the daily amount of food and water intake were measured. Pancreatic tissues were collected for histologic analyses. Pathological assessment and immunohistochemistry analysis were used to determine the effect of metformin on insulitis. Inflammatory cytokines in the pancreas and insulin levels were measured through ELISA analysis. Metformin significantly reduced blood glucose levels and improved aberrant water intake behavior in experimental diabetic mice. No significant differences were observed in terms of body weight and food intake behavior in metformin-treated animals. In the STZ-induced model of diabetes, we found the appearance of pronounced insulitis. However, metformin administration reduced the severity of insulitis assessed by blind pathological scoring. In addition, metformin treatment improved insulin levels in experimental diabetic mice. ELISA assay revealed decreased levels of inflammatory response marker IL-1β and TNF-α in the pancreatic tissues following metformin treatment. Metformin attenuated insulitis in the STZ-induced mice model of diabetes. This islet-protective effect might be partly correlated with the anti-inflammatory action of metformin.