LIVER-SPECIFIC EXPRESSION OF HEPATITIS-B VIRUS IS DETERMINED BY THE COMBINED ACTION OF THE CORE GENE PROMOTER AND THE ENHANCER
LIVER-SPECIFIC EXPRESSION OF HEPATITIS-B VIRUS IS DETERMINED BY THE COMBINED ACTION OF THE CORE GENE PROMOTER AND THE ENHANCER
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DOI：
10.1128/jvi.63.2.919-924.1989
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发表时间：
1989-02-01
影响因子：
5.4
通讯作者：
LAUB, O
中科院分区：
文献类型：
--
作者：
HONIGWACHS, J;FAKTOR, O;LAUB, O
乙型肝炎病毒 (HBV) 增强子和核心基因启动子调节核心基因和聚合酶基因以及 3.5 KB 前基因组 RNA 的表达。 RNA分析和通过携带与异源β-珠蛋白或猿猴病毒40早期启动子连接的HBV增强子的质粒进行的氯霉素乙酰转移酶基因表达证明，HBV增强子在人肝细胞中优先表达3至20倍。核心基因启动子活性被映射到一个 100 个碱基对的片段，该片段被证明足以准确启动转录。该启动子的部分组织特异性通过用含有与异源猿病毒40增强子连接的核心基因启动子的质粒瞬时转染到各种细胞系中来证明。当在HBV增强子的控制下检查HBV核心基因启动子时，存在高度组织特异性，因为仅在分化的人肝细胞中才能观察到活性。这些结果表明，HBV 基因表达的严格组织特异性是由这两个元件的组合作用决定的。
The hepatitis B virus (HBV) enhancer and the core gene promoter regulate the expression of the core and polymerase genes, as well as of the 3.5-kilobase pregenomic RNA. RNA analysis and chloramphenicol acetyltransferase gene expression by plasmids carrying the HBV enhancer linked to the heterologous .beta.-globin or simian virus 40 early promoter demonstrated that the HBV enhancer is 3- to 20-fold preferentially expressed in human liver cells. Core gene promoter activity was mapped to a 100-base-pair fragment which was shown to be sufficient for accurate initiation of transcription. The partial tissue specificity of this promoter was demonstrated by transient transfection into various cell lines with a plasmid containing the core gene promoter linked to the heterologous simian virus 40 enhancer. When the HBV core gene promoter was examined under the control of the HBV enhancer, there was high tissue specificity in that activity could be observed only in differentiated human liver cells. These results suggest that the strict tissue specificity of HBV gene expression is determined by the combinatorial action of these two elements.