Comparison of On-line Single-Capillary and Bridge Capillary Viscometric Detectors for Size Exclusion Chromatography
Comparison of On-line Single-Capillary and Bridge Capillary Viscometric Detectors for Size Exclusion Chromatography
复制标题
用于尺寸排阻色谱的在线单毛细管和桥式毛细管粘度检测器的比较
DOI：
10.1080/10236669708033941
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发表时间：
1997
影响因子：
1.9
通讯作者：
W. Reed
中科院分区：
文献类型：
--
作者：
D. Norwood;W. Reed
摘要对实验室自制的单毛细管粘度计和桥式粘度计进行了系统和全面的比较，将它们集成到一个包含多角度激光散射（MALLS）光度计和差示折光仪的尺寸排阻色谱（SEC）装置中。它表明，适当选择的SEC泵是至关重要的，以实现任何粘度计设计的潜在精度。随机测量误差在单毛细管粘度计中比在桥式粘度计中贡献大一个数量级的误差，并且因此桥式粘度计在任何一次测量中给出显著更高的精度。然而，其他误差来源，如校准误差和从一次运行到下一次运行的随机变化，将桥式粘度计的更高精度降低到仅比单毛细管设计大两倍左右。弥补实验室自制的单毛细管粘度计精度下降的两个优点是其结构紧凑，
Abstract A systematic and thorough comparison is made of a laboratory-built single-capillary viscometer and a bridge viscometer, integrated together into a size exclusion chromatography (SEC) apparatus incorporating a multi-angle laser light scattering (MALLS) photometer and a differential refractometer. It is demonstrated that proper choice of the SEC pump is crucial to realize the potential accuracy of either viscometer design. Random measurement error contributes an order of magnitude greater error in the single-capillary viscometer than in the bridge viscometer, and thus the bridge viscometer gives significantly greater precision in any one measurement. Other sources of error, such as calibration error and stochastic variation from one run to the next, however, reduce the bridge viscometer' s greater precision to only about a factor of two greater than the single-capillary design. Two advantages compensating for the laboratory-built single-capillary viscometer's decreased precision are its compactness...
影响因子：
2.8
作者：
G. Habermehl
通讯作者：
G. Habermehl