Design and validation of a compressive tissue stimulator with high-throughput capacity and real-time modulus measurement capability.
Design and validation of a compressive tissue stimulator with high-throughput capacity and real-time modulus measurement capability.
复制标题
具有高通量能力和实时模量测量能力的压缩组织刺激器的设计和验证。
DOI：
10.1089/ten.tec.2011.0233
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
V. Shastri
中科院分区：
文献类型：
--
作者：
D. Salvetti;C. Pino;Steven G. Manuel;Ian Dallmeyer;Sanjeet V. Rangarajan;T. Meyer;M. Kotov;V. Shastri
机械刺激已被证明会影响工程透明软骨结构的性能，并且与软骨和骨软骨组织的工程相关。迄今为止开发的大多数机械刺激器强调精度，而不是对标准组织培养设备和方案的适应性。在接近天然软骨的工程结构中实现机械特性需要优化复杂变量（刺激类型、方案和双分子信号）。我们提出并验证了一种刺激器设计，该设计侧重于高构建能力、与组织培养塑料器皿的兼容性和方案适应性，以最大限度地提高吞吐量。该设计利用薄力传感器代替称重传感器和线性编码器来验证位置。每个样品的单独力传感器的实现使杨氏模量的测量，同时刺激样品。可拆卸和可互换的聚四氟乙烯柱塞安装使用钕磁铁接触每个样品。柱塞高度和设计的变化可以改变单个样品的应变和力类型。这允许同时评估无数的培养条件和方案。以接触精度和杨氏模量测量范围为关键参数对系统进行了验证。与用千分尺测量相比，该系统的接触精度在结构高度误差1.16%以内。可以测量从生物陶瓷（松质骨，123 MPa）到软凝胶（1%琼脂糖，20 KPa）的生物材料，而无需对设备进行任何修改。测量的准确性以及广泛的模量测试证明了该设备的独特特性，以及在离体培养条件下通过发育阶段使用该设备绘制组织结构（软骨、骨）杨氏模量实时变化的可行性。
Mechanical stimulation has been shown to impact the properties of engineered hyaline cartilage constructs and is relevant for engineering of cartilage and osteochondral tissues. Most mechanical stimulators developed to date emphasize precision over adaptability to standard tissue culture equipment and protocols. The realization of mechanical characteristics in engineered constructs approaching native cartilage requires the optimization of complex variables (type of stimulus, regimen, and bimolecular signals). We have proposed and validated a stimulator design that focuses on high construct capacity, compatibility with tissue culture plastic ware, and regimen adaptability to maximize throughput. This design utilizes thin force sensors in lieu of a load cell and a linear encoder to verify position. The implementation of an individual force sensor for each sample enables the measurement of Young's modulus while stimulating the sample. Removable and interchangeable Teflon plungers mounted using neodymium magnets contact each sample. Variations in plunger height and design can vary the strain and force type on individual samples. This allows for the evaluation of a myriad of culture conditions and regimens simultaneously. The system was validated using contact accuracy, and Young's modulus measurements range as key parameters. Contact accuracy for the system was excellent within 1.16% error of the construct height in comparison to measurements made with a micrometer. Biomaterials ranging from bioceramics (cancellous bone, 123 MPa) to soft gels (1% agarose, 20 KPa) can be measured without any modification to the device. The accuracy of measurements in conjunction with the wide range of moduli tested demonstrate the unique characteristics of the device and the feasibility of using this device in mapping real-time changes to Young's modulus of tissue constructs (cartilage, bone) through the developmental phases in ex vivo culture conditions.
DOI：
10.1115/1.429656
发表时间：
2000-06-01
影响因子：
1.7
作者：
Mauck, RL;Soltz, MA;Ateshian, GA
通讯作者：
Ateshian, GA
影响因子：
--
作者：
Blunk, T;Sieminski, AL;Freed, JE
通讯作者：
Freed, JE