1.Development of an Engineered Photoactive Yellow Protein as a Cross-Linking Junction for Construction of Photoresponsive Protein-Polymer Conjugates
1.Development of an Engineered Photoactive Yellow Protein as a Cross-Linking Junction for Construction of Photoresponsive Protein-Polymer Conjugates
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1.开发工程光活性黄色蛋白作为构建光响应蛋白-聚合物缀合物的交联点
DOI：
10.1002/cptc.201900024
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发表时间：
2019
期刊：
影响因子：
3.7
通讯作者：
Kazushi
中科院分区：
文献类型：
--
作者：
Ui;Mihoko; Miyauchi;Yusuke; Inoue;Masataka; Murakami;Makoto; Araki;Yasuyuki; Wada;Takehiko; Kinbara;Kazushi
基于细菌蓝光受体光敏黄蛋白（PYP），我们开发了一个光敏蛋白模块Hpg 5-PYP，带有5个炔基作为反应位点。我们通过使用共价键合的Hpg 5-PYP与聚（N，N-二甲基丙烯酰胺-co-2-羟基-3-叠氮基丙基甲基丙烯酸酯）（DMA-HAZPMA共聚物）构建了黄色光响应蛋白质-聚合物缀合物。Hpg 5-PYP在缀合物中保留了对蓝光照射的光反应性，并且缀合物在25 °C下用蓝光照射时显示出光响应粘度变化。相比之下，制备用于参考的Hpg 5-PYP和DMA-HAZPMA共聚物的混合物没有显示出粘度对光照射的任何依赖性，表明交联的重要性。
Based on a bacterial blue light photoreceptor, photoactive yellow protein (PYP), we have developed a photoresponsive protein module, Hpg5‐PYP, bearing five alkynyl groups as reactive sites. We constructed a yellow‐colored photoresponsive protein–polymer conjugate by using covalently bonded Hpg5‐PYP with poly(N,N‐dimethylacrylamide‐co‐2‐hydroxy‐3‐azidopropyl methacrylate) (DMA‐HAZPMA copolymer). Hpg5‐PYP retained photoreactivity towards blue‐light irradiation in the conjugate, and the conjugate showed a photoresponsive viscosity change upon irradiation with blue light at 25 °C. In contrast, a mixture of Hpg5‐PYP and DMA‐HAZPMA copolymer prepared for reference did not show any dependency of the viscosity on light irradiation, indicating the importance of crosslinking.