Culture in vector-averaged gravity environment in a clinostat results in detachment of osteoblastic ROS 17/2.8 cells.
Culture in vector-averaged gravity environment in a clinostat results in detachment of osteoblastic ROS 17/2.8 cells.
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发表时间：
1999-12
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通讯作者：
D. Sarkar;T. Nagaya;K. Koga;H. Seo
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作者：
D. Sarkar;T. Nagaya;K. Koga;H. Seo
在空间飞行和改变的重力环境中进行的研究表明，暴露在改变的重力条件下会导致细胞结构和功能的改变。在本研究中，我们使用一个旋转器来产生一个载体平均重力环境，并评估成骨细胞样ROS 17/2.8细胞在50 r.p.m.旋转24至72小时后的反应。我们发现细胞在恒温器培养的前24小时开始分离，但在静止和水平旋转（后者作为湍流，剪切力和振动的控制）中没有分离。24小时时，倾斜旋转下的贴壁细胞数量显著减少(固定培养为2.75 +/- 0.5 × 10(5)，而倾斜旋转培养为2.02 +/- 0.27 × 10(5))，在2%胎牛血清中培养时，19.8%的贴壁细胞呈台番蓝阳性。离体细胞均为台盼蓝阳性。72小时时，三组细胞融合。这些结果表明，在倾斜旋转的前24小时，载体平均重力可能导致成骨细胞死亡。我们假设这种细胞死亡可能在实际太空飞行中观察到的骨质疏松性骨质流失的发病机制中起作用。
Studies carried out in space flights and in altered gravitational environments have shown that exposure to altered gravity conditions results in alterations in cellular structure and function. In the present study, we used a clinostat to generate a vector-averaged gravity environment, and evaluated the responses of osteoblast-like ROS 17/2.8 cells subsequent to rotation at 50 r.p.m from 24 to 72 hr. We found that the cells started to detach during the first 24 hr of culture in clinostat, but not in stationary and horizontal rotation (the latter serving as a control for turbulence, shear forces and vibrations). At 24 hr, there was a significant decrease in the number of adherent cells under clino-rotation (2.75 +/- 0.5 x 10(5) in stationary culture versus 2.02 +/- 0.27 x 10(5) under clino-rotation), and 19.8% of adherent cells were trypan-blue positive when cultured in 2% fetal bovine serum. All the detached cells were trypan-blue positive. At 72 hr, the cells became confluent in all three groups. These results suggest that vector-averaged gravity could cause the death of osteoblasts during the first 24 hr of clino-rotation. We hypothesize that this cell death might play a role in the pathogenesis of osteoporotic bone loss as observed in actual space flight.