Identification and expression of an uncharacterized Ly-6 gene cluster in zebrafish Danio rerio
Identification and expression of an uncharacterized Ly-6 gene cluster in zebrafish Danio rerio
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DOI：
10.1007/s10142-015-0449-9
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发表时间：
2015-06
影响因子：
2.9
通讯作者：
Quanyang Guo;Dongrui Ji;Man Wang;Shicui Zhang;Hongyan Li
中科院分区：
文献类型：
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作者：
Quanyang Guo;Dongrui Ji;Man Wang;Shicui Zhang;Hongyan Li
在大多数后生动物中发现的Ly-6/uPAR/CD59/神经毒素超家族（Ly-6SF）已被证明在不同的生物过程中发挥重要作用，包括免疫、细胞粘附和细胞信号传导。这个超家族的成员含有一个或多个被称为Ly-6/uPAR （LU）结构域的保守结构域，其中包含8或10个保守的半胱氨酸残基，形成4-5个二硫键。本研究报道了在斑马鱼21号染色体上鉴定出一个由ly21.1、ly21.2、ly21.3、ly21.4、ly21.5、ly21.6和ly21.7七个基因组成的斑马鱼ly -6基因簇及其发育过程中的时空表达模式。这7个基因都具有Ly-6/神经毒素超家族的特征，系统发育分析表明，这些基因与Ly-6家族的其他成员形成一个单一的集群分支，表明这7个基因是通过染色体内基因复制事件进化而来的。然而，推断Ly21.1-7蛋白与其他物种的Ly-6家族蛋白几乎没有同源性，在包括硬骨鱼在内的脊椎动物中没有发现同源性，这暗示Ly21.1-7基因是斑马鱼进化上的新添加。表达分析表明，fly21.1-7基因的母体mrna在发育早期被检测到，但在发育后期，它们表现出组织特异性表达。除了在皮肤离子细胞中表达的ly21.2外，其余6个基因主要在发育中的大脑中表达。
The Ly-6/uPAR/CD59/neurotoxin superfamily (Ly-6SF) identified in most metazoan has been shown to play important roles in different biological processes including immunity, cellular adhesion, and cell signaling. Members of this superfamily contain one or more conserved domains known as Ly-6/uPAR (LU) domain, which harbors 8 or 10 conserved cysteine residues forming 4–5 disulfide bonds. In this study, we reported the identification of a novel zebrafishLy-6gene cluster on chromosome 21, which consists of seven genesly21.1,ly21.2,ly21.3,ly21.4,ly21.5,ly21.6, andly21.7and their spatiotemporal expression pattern during development. All the seven genes possess features typical of theLy-6/neurotoxinsuperfamily, and phylogenetic analysis shows that these genes form a single cluster branching form other members of Ly-6 family, suggesting that the seven genes evolved by an event of intra-chromosome gene duplication. However, deduced Ly21.1-7 proteins share little homology with Ly-6 family proteins from other species, no orthologs are identified in vertebrates, including teleosts, hinting thatly21.1-7genes are evolutionarily a novel addition to zebrafish. Expression analyses show that maternal mRNAs ofly21.1-7genes are detected during early developmental stages, but later in development, they exhibit tissue-specific expression. Except forly21.2which is expressed in the skin ionocytes, all the remaining six genes are mainly expressed in the developing brain.