The Histone Variant His2Av is Required for Adult Stem Cell Maintenance in the Drosophila Testis
The Histone Variant His2Av is Required for Adult Stem Cell Maintenance in the Drosophila Testis
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DOI：
10.1371/journal.pgen.1003903
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发表时间：
2013-11-01
期刊：
影响因子：
4.5
通讯作者：
Fuller, Margaret T.
中科院分区：
文献类型：
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作者：
Prado, Jose Rafael Morillo;Srinivasan, Shrividhya;Fuller, Margaret T.
许多组织由成体干细胞维持，成体干细胞通过分化取代丢失的细胞，并通过自我更新维持自己的种群。因此，成体干细胞保持其身份的机制对于整个生命中的组织稳态和修复非常重要。在这里，我们表明，组蛋白的变体，His2Av，所需的细胞自主维持生殖系和囊肿干细胞在果蝇睾丸。ATP依赖性染色质重塑因子多米诺也需要在这个组织中的成体干细胞的维护可能通过调节His2Av纳入染色质。有趣的是，虽然His2Av的表达是普遍存在的，但其功能对于种系和囊肿细胞分化是不确定的，这表明这种非经典组蛋白在维持这些谱系中的干细胞状态中具有特定作用。
Many tissues are sustained by adult stem cells, which replace lost cells by differentiation and maintain their own population through self-renewal. The mechanisms through which adult stem cells maintain their identity are thus important for tissue homeostasis and repair throughout life. Here, we show that a histone variant, His2Av, is required cell autonomously for maintenance of germline and cyst stem cells in the Drosophila testis. The ATP-dependent chromatin-remodeling factor Domino is also required in this tissue for adult stem cell maintenance possibly by regulating the incorporation of His2Av into chromatin. Interestingly, although expression of His2Av was ubiquitous, its function was dispensable for germline and cyst cell differentiation, suggesting a specific role for this non-canonical histone in maintaining the stem cell state in these lineages.