forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster.
forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster.
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叉状蛋白质是果蝇发育中刚毛中存在的纤维束的组成部分。
DOI：
10.1093/genetics/136.1.173
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Corces,VG
中科院分区：
文献类型：
--
作者：
Petersen,NS;Lankenau,DH;Mitchell,HK;Young,P;Corces,VG
黑腹果蝇（Drosophila melanogaster）的叉状（f）基因编码六种不同的转录物，长度为6.4、5.6、5.4、2.5、1.9和1.1 kb。这些转录物通过使用替代启动子而产生。一个多克隆抗体提出了对所有的分叉编码的产品共同的结构域已被用来识别分叉蛋白的二维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶和果蝇蛹组织。抗体染色纤维束存在于刚毛细胞约15小时，在正常蛹的发展。电子显微镜显示，这些纤维是目前从40至53小时的野生型苍蝇的刚毛，但在无效的f36 a突变体缺席。因此，分叉蛋白质似乎是刚毛纤维的重要组成部分。f36 a突变的表型可以通过包含2.5、1.9和1.1 kb转录本编码区的分叉位点的13 kb片段来拯救，这表明由三个大的分叉RNA编码的蛋白质在刚毛发育期间被降解。增加含有13-kb片段的P[w+，f+]构建体的拷贝数诱导了一种多态性刚毛表型，其严重程度与存在的P[w+，f+]的拷贝数相关。这些结果表明，分叉的蛋白质之间的比例，或分叉的产品和其他组件的纤维之间的变化，导致异常组装的刚毛形态发生所需的纤维状细胞质结构。
The forked (f) gene of Drosophila melanogaster encodes six different transcripts 6.4, 5.6, 5.4, 2.5, 1.9, and 1.1 kb long. These transcripts arise by the use of alternative promoters. A polyclonal antibody raised against a domain common to all of the forked-encoded products has been used to identify forked proteins on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and in Drosophila pupal tissues. The antibody stains fiber bundles present in bristle cells for about 15 hr during normal pupal development. Electron microscopy shows that these fibers are present from 40 to 53 hr in bristles of wild-type flies but are absent in the null f36a mutant. The forked protein(s) thus appear to be an essential part of the bristle fibers. The phenotype of the f36a mutation can be rescued by a 13-kb fragment of the forked locus containing the coding regions for the 2.5, 1.9, and 1.1-kb transcripts, suggesting that the proteins encoded by the three large forked RNAs are dispensable during bristle development. Increasing the copy number of a P[w+,f+] construct containing the 13-kb fragment induces a hypermorphic bristle phenotype whose severity correlates with the number of copies of P[w+,f+] present. These results indicate that alterations in the ratios among the forked proteins, or between forked products and other components of the fiber, result in abnormal assembly of the fibrillar cytoplasmic structures necessary for bristle morphogenesis.
DOI：
10.1073/pnas.42.2.73
发表时间：
1956
影响因子：
11.1
作者：
M. M. Green
通讯作者：
M. M. Green