Spatially and temporally regulated expression of myosin heavy chain alternative exons during Drosophila embryogenesis.
Spatially and temporally regulated expression of myosin heavy chain alternative exons during Drosophila embryogenesis.
复制标题
果蝇胚胎发生过程中肌球蛋白重链替代外显子的空间和时间调节表达。
DOI：
10.1016/s0925-4773(00)00549-9
复制
发表时间：
2001
影响因子：
2.6
通讯作者：
Bernstein,SI
中科院分区：
文献类型：
--
作者：
Zhang,S;Bernstein,SI
我们使用选择性外显子特异性探针来测定黑腹果蝇胚胎中肌球蛋白重链（MHC）亚型编码转录本的积累。有六种异构体积聚在体壁肌肉中。横（外）肌与中间肌和内肌表现出不同的主要形式，表明不同的生理特性。成心细胞表达一种体肌转录本；内脏肌肉至少表达两种转录物类型。咽肌积累了独特的Mhc转录本，表明独特的收缩能力。内脏和躯体肌肉的Mhc转录开始于12期，但在成心细胞中则晚至15期。这是昆虫胚胎发生过程中肌球蛋白异构体定位的首次研究，并为转基因和生化实验奠定了基础，旨在确定MHC结构域如何调节肌肉生理。
We used alternative exon-specific probes to determine the accumulation of transcripts encoding myosin heavy chain (MHC) isoforms in Drosophila melanogaster embryos. Six isoforms accumulate in body wall muscles. Transverse (external) muscles express a different major form than intermediate and internal muscles, suggesting different physiological properties. Cardioblasts express one of the somatic muscle transcripts; visceral muscles express at least two transcript types. The pharyngeal muscle accumulates a unique Mhc transcript, suggesting unique contractile abilities. Mhc transcription begins in stage 12 in visceral and somatic muscles, but as late as stage 15 in cardioblasts. This is the first study of myosin isoform localization during insect embryogenesis, and forms the basis for transgenic and biochemical experiments designed to determine how MHC domains regulate muscle physiology.