CE-MALDI interface based on inkjet technology
CE-MALDI interface based on inkjet technology
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DOI：
10.1002/elps.200900414
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发表时间：
2009-12-01
期刊：
影响因子：
2.9
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Vannatta, Michael W.;Whitmore, Colin D.;Dovichi, Norman J.
使用喷墨打印阀和喷嘴将基质和样品从毛细管电泳管输送到MALDI板上。通过分离一组标准多肽对该系统进行了评价。这种分离在不到3分钟的时间里产生了多达40,000个理论塔板。一台ABI TOF-TOF仪器的检出限为500amol，所有ABI Q-TOF仪器的检出限为2fmoL，胰酶消化α-乳清蛋白的覆盖率在2.5min内达到70%以上。
An ink jet printer valve and a nozzle were used to deliver matrix and sample from an electrophoresis capillary onto a MALDI plate. The system was evaluated by the separation of a set of standard peptides. That separation generated up to 40 000 theoretical plates in less than 3 min. Detection limits were 500 amol for an ABI TOF-TOF instrument and 2 fmol for all ABI Q-TOF instrument Over 70% coverage was obtained for the tryptic digest of alpha-lactalbumin in less than 2 5 min.