HISTOPATHOLOGY OF VIRUS-LIKE PARTICLES IN HELIOTHIS SPP
HISTOPATHOLOGY OF VIRUS-LIKE PARTICLES IN HELIOTHIS SPP
复制标题
DOI：
10.1016/0022-2011(83)90224-0
复制
发表时间：
1983-01-01
影响因子：
3.4
通讯作者：
HUDSON, JS
中科院分区：
文献类型：
--
作者：
CARNER, GR;HUDSON, JS
在美国南卡罗来纳州的棉花、大豆和花生田中，发现了感染了病毒样颗粒（VLP）的Heliothis蝇幼虫。受感染的幼虫变得苍白和肿胀，停止进食并存活2-3周。这些幼虫的血淋巴呈乳白色，含有许多球形体，直径从1-20 μ m不等。血淋巴中还含有VLP，呈椭圆形，大小为375倍。125海里。用染病幼虫的粗生理盐水提取液进行的感染性试验表明，病原菌可以通过注射传播，但不能通过血液传播。球体的VLP含量为387倍。149nm)，由2个信封包围，并以簇的形式包装在一起。在脂肪体细胞、表皮上皮细胞、气管细胞以及与体壁和肠道相关的结缔组织中也发现了这些VLP。在肌肉组织或中肠上皮细胞中未发现它们。类似的VLP已经在密西西比州的玉米玉米和美国加利福尼亚州的毛癣菌中发现，但在这些研究中都没有对VLP进行正面鉴定。在形态上，它们似乎不同于任何其他先前描述的昆虫病毒。
Larvae of Heliothis spp. collected from cotton, soybean and peanut fields in South Carolina [USA] were found to be infected with virus-like particles (VLP). Infected larvae became pale and swollen, stopped feeding and remained alive for 2-3 wk. Hemolymph from these larvae was milky and contained numerous spherical bodies ranging in diameter from 1-20 .mu.m. The hemolymph also contained VLP which were oval and measured 375 .times. 125 nm. Infectivity tests with crude saline extracts of infected larvae demonstrated that the pathogen could be transmitted by injection but not per os. The spherical bodies contained VLP (387 .times. 149nm) surrounded by 2 envelopes and packed together in clusters. These VLP were also found in fat body cells, cuticular epithelial cells, tracheal cells and connective tissue associated with the body wall and the gut. They were not found in muscle tissue or in midgut epithelial cells. Similar VLP have been found in H. zea from Mississippi and Trichoplusia ni from California [USA], but a positive identification of the VLP has not been made in any of these studies. Morphologically they appear to be distinct from any other previously described insect viruses.