Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA.
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DOI：
10.1016/j.stem.2010.08.012
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发表时间：
2010-11-05
期刊：
影响因子：
23.9
通讯作者：
Rossi, Derrick J.
中科院分区：
文献类型：
--
作者：
Warren, Luigi;Manos, Philip D.;Ahfeldt, Tim;Loh, Yuin-Han;Li, Hu;Lau, Frank;Ebina, Wataru;Mandal, Pankaj K.;Smith, Zachary D.;Meissner, Alexander;Daley, George Q.;Brack, Andrew S.;Collins, James J.;Cowan, Chad;Schlaeger, Thorsten M.;Rossi, Derrick J.
诱导多能茎（IPS）细胞的临床应用受到IPS推导的低效率的限制，并且大多数方案将基因组修改以实现细胞重编程的事实。此外，缺乏将患者特异性IPS细胞的命运引导到临床上有用的细胞类型的安全和有效手段。在这里，我们描述了一种简单的，非整合的策略，用于基于修改的合成mRNA的给药，以克服先天的抗病毒反应。我们表明，这种方法可以通过大大超过已建立的方案的效率来重新编程多种人类细胞类型以多能性。我们进一步表明，相同的技术可用于有效地将RNA诱导的多能茎（RIPS）细胞的分化转化为末端分化的肌源性细胞。该技术代表了对体细胞重编程和指导细胞命运的安全，有效的策略，该命运对基础研究，疾病建模和再生医学具有广泛的适用性。
Clinical application of induced pluripotent stem (iPS) cells is limited by the low efficiency of iPS derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover, safe and effective means of directing the fate of patient-specific iPS cells towards clinically useful cell types are lacking. Here we describe a simple, non-integrating strategy for reprogramming cell fate based on administration of synthetic mRNA modified to overcome innate anti-viral responses. We show that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols. We further show that the same technology can be used to efficiently direct the differentiation of RNA-induced pluripotent stem (RiPS) cells into terminally differentiated myogenic cells. This technology represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling and regenerative medicine.
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影响因子：
12.4
作者：
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通讯作者：
Weissman, Drew
影响因子：
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作者：
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影响因子：
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DOI：
10.1016/j.bbrc.2005.03.067
发表时间：
2005-05-13
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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