Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L
Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L
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DOI：
10.1038/s41598-020-66452-5
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发表时间：
2020-06-11
影响因子：
4.6
通讯作者：
Muturi, Ephantus J.
中科院分区：
文献类型：
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作者：
Juma, Elijah O.;Allan, Brian F.;Muturi, Ephantus J.
杀虫剂通常会污染幼蚊栖息的水生环境。然而，它们在塑造蚊子微生物群中的作用尚不清楚。我们假设，阿特拉津、氯菊酯和马拉硫磷的环境相关浓度将介导蚊子肠道细菌群落结构的转变，因为它们对水生细菌群落有毒性作用，并通过丰富易受影响的分类单元上的农药降解细菌群落来减少蚊子肠道细菌多样性。使用 Illumina MiSeq 对 16S rRNA 基因的 V3-V4 高变区进行测序，分别表征两种蚊子幼虫和成虫阶段的微生物群落以及经每种农药处理的微观世界的水样。细菌群落组成因样品类型（幼虫阶段与成虫阶段）和水采样日期（第 3 天与第 7 天）而异，但不因农药处理而异。在幼虫阶段，细菌 OTU 丰富度在暴露于马拉硫磷的样品中最高，在氯菊酯中居中，在对照中最低。与所有处理的成虫阶段相比，幼虫阶段的细菌丰富度显着更高。这项研究为未来研究评估蚊子微生物对接触化学农药的反应以及对蚊子生态的可能影响提供了基础。
Pesticides commonly contaminate the aquatic environments inhabited by mosquito juveniles. However, their role in shaping the mosquito microbiota is not well understood. We hypothesized that environmentally relevant concentrations of atrazine, permethrin and malathion will mediate a shift in the mosquito gut bacterial community structure due to their toxic effect on the aquatic bacterial communities, and reduce mosquito gut bacterial diversity by enriching pesticide-degrading bacterial communities over susceptible taxa. Illumina MiSeq sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene was used to characterize the microbial communities of larval and adult stages of the two mosquito species and the water samples from microcosms treated with each of the pesticides, separately. Bacterial community composition differed by sample type (larval stage vs. adult stage) and water sampling date (day 3 vs. day 7), but not by pesticide treatment. In larval stages, bacterial OTU richness was highest in samples exposed to malathion, intermediate in permethrin, and lowest in controls. Bacterial richness was significantly higher in larval stages compared to adult stages for all treatments. This study provides a primer for future studies evaluating mosquito microbial responses to exposures to chemical pesticides and the possible implications for mosquito ecology.