Comparison of NxTAG Respiratory Pathogen Panel and Anyplex II RV16 Tests for Multiplex Detection of Respiratory Pathogens in Hospitalized Children
Comparison of NxTAG Respiratory Pathogen Panel and Anyplex II RV16 Tests for Multiplex Detection of Respiratory Pathogens in Hospitalized Children
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DOI：
10.1128/jcm.01243-16
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发表时间：
2016-12-01
影响因子：
9.4
通讯作者：
Munoz-Almagro, Carmen
中科院分区：
文献类型：
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作者：
Brotons, Pedro;Henares, Desiree;Munoz-Almagro, Carmen
多重分子技术可以快速、方便地检测引起儿童急性呼吸道感染的多种呼吸道病毒和细菌。然而，目前可用的技术在性能上表现出很大的差异。我们试图比较新的多重NxTAG呼吸道病原体面板(RPP)RUO试验和常规多重Anyplex II RV16试验在从18岁住院的急性下呼吸道感染的非特异性症状中收集的呼吸道标本中的诊断准确性。2015年6月至11月期间，对转诊儿童医院Sant Joan de Deu(西班牙巴塞罗那)预期收集的鼻咽抽吸物进行了平行测试。评估了两种测试之间的符合值和卡帕系数。为了解决不一致的结果，进行了双向测序。用这两种技术对319个样品进行了分析。结果符合者268例(84.0%)。阳性符合率从83.3%到100%，而除肠道病毒/鼻病毒(EV/RV；94.4%)外，所有指标的阴性符合率均超过99%。卡帕系数在0.83~1.00之间。差异分析证实了NxTAG RPP RUO结果的66.0%。共检出260株病毒，其中EV/RV(n=105，40.4%)是最流行的目标。病毒混合感染44例(14.2%)。此外，NxTAG RPP Ruo在7个样本中检测到单一细菌和病毒-细菌混合感染。对于导致儿童急性呼吸道感染的主要病毒，NxTAG RPP Ruo与Anyplex II RV16显示出高度的阳性和阴性一致性，并增加了检测一些呼吸道细菌的能力。
Multiplex molecular techniques can detect a diversity of respiratory viruses and bacteria that cause childhood acute respiratory infection rapidly and conveniently. However, currently available techniques show high variation in performance. We sought to compare the diagnostic accuracy of the novel multiplex NxTAG respiratory pathogen panel (RPP) RUO test versus a routine multiplex Anyplex II RV16 assay in respiratory specimens collected from children< 18 years of age hospitalized with nonspecific symptoms of acute lower respiratory infection. Parallel testing was performed on nasopharyngeal aspirates prospectively collected at referral Children's Hospital Sant Joan de Deu (Barcelona, Spain) between June and November 2015. Agreement values between the two tests and kappa coefficients were assessed. Bidirectional sequencing was performed for the resolution of discordant results. A total of 319 samples were analyzed by both techniques. A total of 268 (84.0%) of them yielded concordant results. Positive percent agreement values ranged from 83.3 to 100%, while the negative percent agreement was more than 99% for all targets except for enterovirus/rhinovirus (EV/RV; 94.4%). Kappa coefficients ranged from 0.83 to 1.00. Discrepancy analysis confirmed 66.0% of NxTAG RPP RUO results. A total of 260 viruses were detected, with EV/RV (n = 105, 40.4%) being the most prevalent target. Viral coinfections were found in 44 (14.2%) samples. In addition, NxTAG RPP RUO detected single bacterial and mixed viral-bacterial infections in seven samples. NxTAG RPP RUO showed high positive and negative agreement with Anyplex II RV16 for main viruses that cause acute respiratory infections in children, coupled with an additional capability to detect some respiratory bacteria.