Zebrafish prmt7 negatively regulates antiviral responses by suppressing the retinoic acid-inducible gene-I-like receptor signaling.
Zebrafish prmt7 negatively regulates antiviral responses by suppressing the retinoic acid-inducible gene-I-like receptor signaling.
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斑马鱼 prmt7 通过抑制视黄酸诱导基因 I 样受体信号传导来负向调节抗病毒反应。
DOI：
10.1096/fj.201902219r
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Xiao Wuhan
中科院分区：
文献类型：
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作者：
Zhu Junji;Liu Xing;Cai Xiaolian;Ouyang Gang;Fan Sijia;Wang Jing;Xiao Wuhan
精氨酸甲基化是组蛋白和非组蛋白中的翻译后修饰，可以影响许多细胞活性。蛋白质精氨酸甲基转移酶7（Prmt 7）是一种III型精氨酸甲基转移酶，催化组蛋白形成稳定的单甲基精氨酸。然而，PRMT 7在病毒诱导的先天免疫信号传导中的作用在很大程度上仍然未知。我们证明斑马鱼prmt 7可以被鲤鱼春季病毒血症病毒（SVCV）和草鱼呼肠孤病毒（GCRV）感染所抑制。prmt 7的过度表达抑制了部分依赖于prmt 7精氨酸甲基转移酶活性的细胞抗病毒反应。一致的是，prmt 7-null斑马鱼对SVCV或GCRV感染更具抵抗力，在病毒感染后表现出关键抗病毒基因的表达增强和肝脏和肾脏中坏死细胞较少。此外，我们还建立了一个研究草鱼出血病的斑马鱼模型。我们的研究结果表明，通过抑制RIG-I-like受体信号传导，斑马鱼prmt 7负调节抗病毒反应，表明prmt 7及其精氨酸甲基转移酶活性在先天免疫中的重要作用。
Arginine methylation is a post‐translational modification in histone and nonhistone proteins that can affect numerous cellular activities. Protein arginine methyltransferase 7 (Prmt7), a type III arginine methyltransferase, catalyzes the formation of stable monomethylarginines of histones. The role of PRMT7 in virus‐induced innate immunity signaling, however, remains largely unknown. We demonstrate that zebrafishprmt7could be inhibited by spring viremia of carp virus (SVCV) and grass carp reovirus (GCRV) infection. The overexpression ofprmt7suppresses cellular antiviral responses that are partially dependent on the arginine methyltransferase activity ofprmt7. Consistently,prmt7‐null zebrafish were more resistant to SVCV or GCRV infection, exhibiting enhanced expression of key antiviral genes and fewer necrotic cells in the liver and kidney upon viral infection. Furthermore, we established a zebrafish model to investigate grass carp hemorrhagic disease. Our findings suggest that by suppressing the RIG‐I‐like receptors signaling, zebrafishprmt7negatively regulates antiviral responses, indicating the vital role ofprmt7and its arginine methyltransferase activity in innate immunity.