An upstream promoter element of the Acanthamoeba castellanii TBP gene binds a DNA sequence specific transcription activating protein, TPBF.
An upstream promoter element of the Acanthamoeba castellanii TBP gene binds a DNA sequence specific transcription activating protein, TPBF.
复制标题
卡氏棘阿米巴 TBP 基因的上游启动子元件结合 DNA 序列特异性转录激活蛋白 TPBF。
DOI：
10.1093/nar/21.18.4321
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发表时间：
1993
影响因子：
14.9
通讯作者：
Bateman,E
中科院分区：
文献类型：
--
作者：
Liu,F;Bateman,E
We have characterized a positive-acting element In the upstream portion of the Acanthamoeba TBP gene promoter. The 27 bp element (TPE), located within the promoter between -97 and -70, stimulates transcription in an orientation Independent fashion and tolerates modest changes in its distance from the TATA box. The TPE does not, however, function synerglstlcally nor when positioned 3000 bp 5' or 260 base pairs 3' of the transcription start site. The TPE binds a DNA sequence-dependent factor, TPBF, which we have partly purified. TPBF was characterized using In vitro transcription, DNase I foot print ing, methylatlon Interference and electrophoretlc mobility shift assays. TPBF does not have a counterpart in HeLa cells, but nonetheless strongly stimulates transcription of the Acanthamoeba TBP gene in mammalian extracts. Our results also suggest that there are additional positively and negatively acting elements within the TBP gene promoter, for which a model Is presented.
