Genetic patterning in the adult capitate antenna of the beetle Tribolium castaneum
Genetic patterning in the adult capitate antenna of the beetle Tribolium castaneum
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DOI：
10.1016/j.ydbio.2008.10.047
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发表时间：
2009-03-01
影响因子：
2.7
通讯作者：
Jockusch, Elizabeth L.
中科院分区：
文献类型：
--
作者：
Angelini, David R.;Kikuchi, Moto;Jockusch, Elizabeth L.
与保存完好的腿结构相比，昆虫的触角结构差异很大。赤粉甲成虫的头状触角由11条组成，分成四个不同的形态区域(花梗、柄)。在这里，我们报告了使用RNA干扰来检测21个基因在卡氏锥虫触角变态过程中的功能。与发育模式物种果蝇相关的保守功能基因包括远端信号和EGF信号(触角生长)、无刺(确定触角特性)和Notch信号通路(触角生长、关节形成和感觉刚毛发育)。然而，许多基因的功能与果蝇模型预测的不同。除了保守的GAP表型外，腊肠犬的枯竭还将索状物转变为棒状身份。远端或同胸的耗尽不会导致天线到腿部的转变。LIM1仅用于发展椎弓根关节。奇数跳过相关基因的耗尽导致整个神经索的丧失，而Spalt、圆圆的、无刺的和腊肠影响较小的区域。生长和关节的形成在杆部是发育相关的，而在球茎中不是。俱乐部内部的联合形成需要Brica-brac、aristess、Apterous和pdm。根据卡氏锥虫触角发育模型对基因功能进行了讨论。该模型提供了与黑腹毛虫的天线发育知识的对比，洞察了天线发育的可能的祖先模式，并提供了一个考虑不同天线形态的框架。(C)2008 Elsevier Inc.保留所有权利。
Antenna structure varies widely among insects, in contrast to the well-conserved structure of legs. The adult capitate antenna of the red flour beetle, Tribolium castaneum, is composed of eleven articles, organized into four distinct morphological regions (scape, pedicel, funicle and club). Here, we report the use of RNA interference to examine the functions of 21 genes during antenna metamorphosis in T castaneum. Genes with conserved functions relative to the developmental model species Drosophila melanogaster include Distal-less and EGF signaling (antennal growth), spineless (determination of antenna] identity) and the Notch signaling pathway (antenna] growth, joint formation, and sensory bristle development). However, the functions of many genes differed from those predicted from the Drosophila model. In addition to a conserved gap phenotype, depletion of dachshund transformed funicle articles toward club-like identity. Depletion of Distal-less or homothorax did not cause antenna-to-leg transformation. Lim1 was required only for development of the scape-pedicle joint. Depletion of odd-skipped-related genes led to the loss of the entire funicle, while spalt, rotund, spineless, and dachshund affected smaller regions. Growth and joint formation were linked developmentally in the funicle, but not in the club. joint formation within the Club required bric-a-brac, aristaless, apterous, and pdm. Gene functions are discussed in terms of a model of antenna development in T castaneum. This model provides a contrast to knowledge of antenna development in D. melanogaster, insight into the likely ancestral mode of antenna development, and a framework for considering diverse antenna morphologies. (C) 2008 Elsevier Inc. All rights reserved.