Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent
Field study of the bioremediation of oil-contaminated Dalian beach by marine oil-degrading bacterial agent
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作者：
C. Lei
【摘要】：将海洋石油降解菌群DC10制备成石油降解剂,并将其应用于大连海滩原油污染的生物修复中。分别在潮间带和潮上带进行了现场研究,通过评价C17/藿烷、C18/藿烷、总石油烷烃和总石油芳烃的下降情况来考察石油降解剂的效果。在实验室条件下，DC10的降解率远高于单一的复合DC10降解剂。与无菌对照相比，复合DC10的降解率在7天内提高了60%，油中大部分烷烃和芳烃消失。在大连海滩石油污染的现场研究中，证明添加必需营养盐的石油降解剂是有效的。在潮间带样地，与自然风化相比，生物修复处理的C17/藿烷和C18/藿烷的降解率在12 d内分别增加了约40%和30%。而生物修复处理的总烷烃和总芳烃的降解率在12 d内增加了约80%和72%。以潮上带图为例，C17/藿烷和C18/藿烷的降解率85 d时生物修复处理的降解率仅略高于自然风化，而85 d时生物修复处理的总烷烃和总芳烃降解率分别增加了约30%和20%。
A marine oil-degrading bacterial consortium DC10 was prepared to an oil-degrading agent and further applied to the bioremediation of crude oil-contaminated Dalian beach.Field studies were carried out at the intertidal zone and supratidal zone respectively,and the effects of the oil-degrading agent were investigated by evaluating the decline of C17/hopane,C18/hopane,total petroleum alkanes and total petroleum aromatics.The oil-degrading consortium DC10 exhibited much higher degradation rates than the single degrader comprising consortium DC10 under laboratory conditions.Compared to the sterile control,the degradation rates of consortium DC10 increased by 60% in 7 days,and most alkanes and aromatics in oil were disappeared.The oil-degrading agent supplemented with essential nutrient salts was proved to be effective in the field study on the bioremediation of oil-contaminated Dalian beach.At the intertidal zone plot,the C17/hopane and C18/hopane degradation rates of bioremediation treatment increased by approximately 40% and 30% in 12 d,respectively,compared to the natural weathering.While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 80% and 72% in 12 d.In the case of supratidal zone plot,the C17/hopane and C18/hopane degradation rates of bioremediation treatment was only slightly higher than natural weathering in 85 d.While the total alkanes and total aromatics degradation rates of bioremediation treatment increased by approximately 30% and 20% in 85 d,respectively.