Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken.
Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken.
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DOI：
10.1371/journal.pone.0171642
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Oba A
中科院分区：
文献类型：
--
作者：
Costa MC;Bessegatto JA;Alfieri AA;Weese JS;Filho JA;Oba A
有时会给食用动物服用低剂量的抗菌剂，以促进更快的生长。然而，这些药物改善表现的机制尚不完全清楚。本研究旨在探讨杆菌肽锌（55g/吨）、恩拉霉素（10g/吨）的影响；哈喹诺®（30克/吨）；与对照组相比，维吉尼亚霉素（16.5克/吨）和阿维霉素（10克/吨）对肉鸡盲肠微生物群的影响。到达实验单位的 624 只雏鸡 (Cobb 500) 被随机分配到每个处理中，每个处理重复四次。每次处理 16 只动物 (n = 96) 的盲肠内容物用于提取 DNA 并使用 Illumina 技术对 16S rRNA 基因的 V4 区域进行测序。与对照组相比，抗菌药物的使用引起了盲肠微生物群成员的显着变化，但没有引起结构的显着变化，这表明对该环境中存在的数量较少的细菌种类的影响更大。哈喹诺是唯一不影响微生物成员的药物。厚壁菌门是所有群体盲肠中存在的主要细菌门。处理组动物与对照组主要门相对丰度差异无统计学意义（均P>0.05）。恩拉霉素治疗与未分类的厚壁菌门、梭状芽孢杆菌 XI、未分类的消化链球菌科的丰度降低和相对丰度降低相关（所有 P<0.001），以及梭菌 XIVb 的丰度增加（P = 0.004）和厌氧孢杆菌属。 (P = 0.015)，以及用杆菌肽处理具有较高的 Bilophila spp 相对丰度。 （P = 0.004）。确定了几种细菌属来代表每种药物的使用。这项研究使用高通量测序来表征受控条件下几种抗菌药物对肉鸡的影响，并为目前关于 AGP 如何影响鸡盲肠微生物群的知识增添了新的见解。
Antimicrobials are sometimes given to food animals at low doses in order to promote faster growth. However, the mechanisms by which those drugs improve performance are not fully understood. This study aimed to investigate the impact of zinc bacitracin (55g/ton), enramycin (10g/ton); halquinol® (30g/ton); virginiamycin (16,5g/ton) and avilamycin (10g/ton) on the cecal microbiota of broiler chicken, compared to a control group. Six hundred and twenty four chicks (Cobb 500) arriving to an experimental unit were randomly assigned into each treatment with four repetitions per treatment. The cecal content of 16 animals per treatment (n = 96) was used for DNA extraction and sequencing of the V4 region of the 16S rRNA gene using Illumina technology. The use of antimicrobials induced significant changes in membership but not in structure of the cecal microbiota compared to the control group, suggesting a greater impact on the less abundant species of bacteria present in that environment. Halquinol was the only drug that did not affect microbial membership. Firmicutes comprised the major bacterial phylum present in the cecum of all groups. There was no statistical difference in relative abundances of the main phyla between treated animals and the control group (all P>0.05). Treatment with enramycin was associated with decreased richness and with lower relative abundance of unclassified Firmicutes, Clostridium XI, unclassified Peptostreptococcaceae (all P<0.001) and greater abundance of Clostridium XIVb (P = 0.004) and Anaerosporobacter spp. (P = 0.015), and treatment with bacitracin with greater relative abundance of Bilophila spp. (P = 0.004). Several bacterial genera were identified as representative of usage of each drug. This study used high throughput sequencing to characterize the impact of several antimicrobials in broiler chicken under controlled conditions and add new insights to the current knowledge on how AGPs affect the cecal microbiota of chicken.