Manufacture of multimicrotubule chitosan nerve conduits with novel molds and characterization in vitro
Manufacture of multimicrotubule chitosan nerve conduits with novel molds and characterization in vitro
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DOI：
10.1002/jbm.a.30593
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发表时间：
2006-04-01
影响因子：
4.9
通讯作者：
Zhang, XF
中科院分区：
文献类型：
--
作者：
Ao, Q;Wang, AJ;Zhang, XF
采用新型模具和热致相分离技术制备了多微管壳聚糖导管（M-导管）。制备了内径为1-5 mm、壁厚为0.2- 1.0mm的壳聚糖空心导管（H型导管），并采用由多孔泡沫塑料绝缘底座和不锈钢盖板组成的新型模具制备了M型导管。简而言之，将相应的H-导管直立插入到泡沫聚苯乙烯底座的孔中，并填充壳聚糖溶液，然后快速覆盖预冷的不锈钢盖板，然后放置在冰箱中。在导管周围设置发泡胶隔热底座，可减少导管侧壁的热传递。然后，在从壳聚糖导管的顶端到底端的单向温度梯度的存在下，单轴地发生逐渐的相分离。然后将相分离的聚合物/溶剂体系在冷冻干燥器中干燥。通过调节聚合物浓度和冷却温度来控制微管直径。体外性能测试表明，该多微管壳聚糖导管具有良好的机械强度、微管直径分布、孔隙率、溶胀性、生物降解性和神经细胞亲和性，具有作为神经组织工程支架的潜力。(c)2005 Wiley Periodicals，Inc.
Multimicrotubule chitosan conduits (M-conduits) were fabricated using novel molds and a thermal-induced phase-separation technique. Hollow chitosan conduits (H-conduits) with an inner diameter of 1-5 mm and a wall thickness of 0.2-1.0 mm were made, and then a novel mold composed of a styrofoam insulating pedestal with several holes and a stainless steel cover plate was used to make M-conduits. In brief, corresponding H-conduits were inserted upright into the holes of the styrofoam pedestal, and filled with chitosan solution, then rapidly covered with the precooled stainless steel cover plate, and then placed in a freezer. The styrofoam insulating pedestal enclosing the conduits could reduce the heat transfer through the side wall of the conduits. Gradual phase separation then occurred uniaxially in the presence of a unidirectional temperature gradient from the top end to the bottom end of the chitosan conduits. The phase-separated polymer/solvent systems were then dried in a freeze-dryer. The microtubule diameters were controlled by adjusting the polymer concentration and cooling temperature. In vitro characterization demonstrated that the mold-based multimicrotubule chitosan conduits possessed suitable mechanical strength, microtubule\ diameter distribution, porosity, swelling, biodegradability, and nerve cell affinity, and so they showed potential for application as nerve tissue engineering scaffolds. (c) 2005 Wiley Periodicals, Inc.