New Populations of Sclerotinia sclerotiorum from Lettuce in California and Peas and Lentils in Washington.
New Populations of Sclerotinia sclerotiorum from Lettuce in California and Peas and Lentils in Washington.
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DOI：
10.1094/phyto-97-4-0470
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发表时间：
2007-03
期刊：
影响因子：
3.2
通讯作者：
G. Malvárez;I. Carbone;N. Grünwald;K. Subbarao;Michelle Schafer;L. Kohn
中科院分区：
文献类型：
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作者：
G. Malvárez;I. Carbone;N. Grünwald;K. Subbarao;Michelle Schafer;L. Kohn
摘要根据快速进化标记(DNA指纹和菌丝亲和性)和跨越快速和缓慢进化范围的多位点DNA序列的分析，已推断出北美4个核盘菌种群。每个种群被定义为同种个体的杂交单位，共享共同的近亲祖先，并出现在独特的进化事件中。本研究应用这一标准将菌核菌种群的特征扩展到美国西部。核盘菌的分离物(N=294)代表了3个遗传分化的种群：加利福尼亚州(CA，生菜)、华盛顿(华盛顿州，豌豆/小扁豆)和安大略省(ON，生菜)。CA是北美迄今被抽样的最多样化的种群。在ON和WA中检测到克隆性。在这些人群中没有常见的DNA指纹。基于指纹的关联指数(I(A))，CA比其他种群更接近于零(0)。加州的高多样性和标记缺乏关联性既与遗传交换和重组相一致，也与种群规模大和遗传变异高相一致。3个DNA序列基因座之间的位点内和位点间的冲突与重组一致。合并的IGS家系确认了亚群，并显示CA比Wa或On更古老。组合数据的最近邻统计确认了所有现有和先前定义的种群之间的细分。所有分离株同时具有MAT1-1和MAT1-2，符合均一的同质性。
ABSTRACT Four populations of Sclerotinia sclerotiorum in North America were inferred previously, based on analyses of both rapidly evolving markers (DNA fingerprint and mycelial compatiblity), and multilocus DNA sequence spanning the range between fast and slow evolution. Each population was defined as an interbreeding unit of conspecific individuals sharing a common recent ancestor and arising in a unique evolutionary event. The present study applies this standard to extend characterization of S. sclerotiorum populations to the Western United States. Isolates of S. sclerotiorum (N = 294) were determined to represent three genetically differentiated populations: California (CA, lettuce), Washington (WA, pea/lentil), and Ontario (ON, lettuce). CA was the most diverse population yet sampled in North America. Clonality was detected in ON and WA. No DNA fingerprints were common among the populations. The index of association (I(A)), based on fingerprint, was closer to zero (0) for CA than it was for the other populations. High diversity and lack of association of markers in California are consistent either with genetic exchange and recombination, or with large population size and high standing genetic variation. Intra- and interlocus conflict among three DNA sequence loci was consistent with recombination. The coalescent IGS genealogy confirmed subdivision and showed CA to be older than WA or ON. The Nearest Neighbor statistic on combined data confirmed subdivision among all present and previously defined populations. All isolates had both MAT1-1 and MAT1-2, consistent with uniform homothallism.