Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2.
Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2.
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DOI：
10.1016/j.isci.2023.107690
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发表时间：
2023-09-15
期刊：
影响因子：
5.8
通讯作者：
Jaenisch, Rudolf
中科院分区：
文献类型：
--
作者：
Flamier, Anthony;Bisht, Punam;Richards, Alexsia;Tomasello, Danielle L.;Jaenisch, Rudolf
自二零一九年以来，COVID-19已影响数十亿人，并引发重大医疗危机。随着死亡人数的增加和更多传染性变异的出现，更好地了解致病病毒SARS-CoV-2的生物学至关重要。周围神经病变作为一种特定的COVID-19症状出现在疾病的后期阶段。为了了解SARS-CoV-2对周围神经系统的影响，我们从诱导多能干细胞中产生了人类感觉神经元，我们用SARS-CoV-2菌株WA 1/2020和变体delta和omicron感染。使用单细胞RNA测序，我们发现人类感觉神经元可以被SARS-CoV-2感染，但不能产生感染性病毒。我们的数据表明，感觉神经元可以被SARS-CoV-2的原始WA 1/2020毒株以及δ和omicron变体感染，但原始毒株和变体之间的感染性不同。感觉神经元可被SARS-CoV-2感染WA 1/2020毒株以及δ和omicron变异株之间的易感性不同受感染的感觉神经元合成SARS-CoV-2 RNA，但不产生子代病毒粒子
COVID-19 has impacted billions of people since 2019 and unfolded a major healthcare crisis. With an increasing number of deaths and the emergence of more transmissible variants, it is crucial to better understand the biology of the disease-causing virus, the SARS-CoV-2. Peripheral neuropathies appeared as a specific COVID-19 symptom occurring at later stages of the disease. In order to understand the impact of SARS-CoV-2 on the peripheral nervous system, we generated human sensory neurons from induced pluripotent stem cells that we infected with the SARS-CoV-2 strain WA1/2020 and the variants delta and omicron. Using single-cell RNA sequencing, we found that human sensory neurons can be infected by SARS-CoV-2 but are unable to produce infectious viruses. Our data indicate that sensory neurons can be infected by the original WA1/2020 strain of SARS-CoV-2 as well as the delta and omicron variants, yet infectability differs between the original strain and the variants. Sensory neurons can be infected by SARS-CoV-2 Infectability differs between WA1/2020 strain as well as the delta and omicron variants Infected sensory neurons synthesize SARS-CoV-2 RNA but do not produce progeny virion Neuroscience; Cellular neuroscience; Virology
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影响因子：
50.5
作者：
Stewart CA;Gay C;Ramkumar K;Cargill KR;Cardnell R;Nilsson M;Heeke S;Park EM;Kundu S;Diao L;Wang Q;Shen L;Xi Y;Della Corte CM;Kundu K;Gibbons DL;Wang J;Heymach JV;Byers LA
通讯作者：
Byers LA
影响因子：
13.6
作者：
通讯作者：
--
影响因子：
64.5
作者：
Bouhaddou, Mehdi;Memon, Danish;Krogan, Nevan J.
通讯作者：
Krogan, Nevan J.
DOI：
10.1016/j.jbc.2022.101677
发表时间：
2022-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cascarina SM;Ross ED
通讯作者：
Ross ED
影响因子：
82.9
作者：
Kuba K;Imai Y;Rao S;Gao H;Guo F;Guan B;Huan Y;Yang P;Zhang Y;Deng W;Bao L;Zhang B;Liu G;Wang Z;Chappell M;Liu Y;Zheng D;Leibbrandt A;Wada T;Slutsky AS;Liu D;Qin C;Jiang C;Penninger JM
通讯作者：
Penninger JM