HUMORAL AND CELLULAR IMMUNE-RESPONSES TO FASCIOLA-HEPATICA EXPERIMENTAL PRIMARY AND SECONDARY INFECTION IN SHEEP
HUMORAL AND CELLULAR IMMUNE-RESPONSES TO FASCIOLA-HEPATICA EXPERIMENTAL PRIMARY AND SECONDARY INFECTION IN SHEEP
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DOI：
10.1016/0020-7519(95)00039-5
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发表时间：
1995-10-01
影响因子：
4
通讯作者：
BOULARD, C
中科院分区：
文献类型：
--
作者：
CHAUVIN, A;BOUVET, G;BOULARD, C
本文研究了实验性初次或二次感染绵羊的血白细胞变化、血清肝酶水平、淋巴细胞对刀豆蛋白A(ConA)和寄生虫排泄分泌产物(FhESP)的反应以及抗体(Ig G和Ig M)反应(酶联免疫吸附试验和免疫印迹试验)。一次感染组和二次感染组建立的吸虫比例相似，但源自二次感染的吸虫迁移到胆管的速度更快。初次感染后3~13周，患者外周血嗜酸性粒细胞明显增多。PPIW 2产生FhESP特异性IgM，峰值出现在PPIW 3和9-10，FhESP特异性免疫球蛋白从PPIW 2增加到6，之后趋于稳定。Western blotting显示FhESP有12、15、20、24、27、28.5、30、41、51、56、69和156 kDa的12个主要抗原组分，部分非特异性抗原组分已被鉴定。观察到高分子量抗原对低分子量抗原的顺序识别。从PPIW 2~5，FhESP特异性淋巴细胞增殖明显，ConA刺激淋巴细胞增殖作用减弱。PPIW 6二次感染后，免疫应答发生改变。ConA诱导的淋巴细胞增殖反应一过性增强。相反，体液反应，尤其是对早期识别的抗原的反应，以及FhESP特异性淋巴细胞增殖反应的水平和持续时间都降低了。
Blood leukocyte changes, serum hepatic enzyme levels, lymphocyte proliferation in response to Concanavalin A (ConA) and to parasitic excretory-secretory products (FhESP), and antibody (IgG and IgM) responses (ELISA and Western blot) were studied in sheep, the natural susceptible host of F. hepatica, during the first 3 months of an experimental primary or secondary infection. The proportion of flukes established was similar in once- and twice-infected groups, but the flukes originating from the secondary infection migrated more rapidly to the bile ducts. Primary infection induced a marked peripheral eosinophilia from 3 to 13 weeks post-primary infection (PPIW). FhESP-specific IgM were produced from PPIW 2 with peaks in PPIW 3 and 9-10; FhESP-specific IgG increased from PPIW 2 to 6 and became stable afterwards. Western blotting revealed 12 major antigenic fractions in FhESP from 12, 15, 20, 24, 27, 28.5, 30, 41, 51, 56, 69 and 156 kDa; some non-specific ones have been characterized. A sequential recognition of higher then lower molecular weight antigens was observed. FhESP-specific lymphocyte proliferation was marked from PPIW 2 to 5. In contrast, ConA stimulation of lymphocytes was decreased. After secondary infection in PPIW 6, immune responses were modified. The ConA-induced lymphocyte proliferation was transitorily increased. In contrast, the humoral response, in particular against the early recognized antigens, and the level and the duration of the FhESP-specific lymphocyte proliferative response, were reduced.