Effect of repeated exercise on urinary 8-hydroxy-deoxyguanosine excretion in humans
Effect of repeated exercise on urinary 8-hydroxy-deoxyguanosine excretion in humans
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DOI：
10.3109/10715769709097821
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发表时间：
1997-01-01
影响因子：
3.3
通讯作者：
SugawaKatayama, Y
中科院分区：
文献类型：
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作者：
Okamura, K;Doi, T;SugawaKatayama, Y
本研究旨在探讨重复运动对10名参加8天训练营的长跑运动员DNA氧化损伤的影响。营地期间的平均跑步距离为30 +/- 3公里/天。尿8-羟基脱氧鸟苷（8-OHdC）排泄量用于评估氧化性DNA损伤。在为期3天的对照期以及整个营地收集尿液样本。在营地前后禁食过夜后抽取血样。与对照期相比，在营地期间尿8-OHdG排泄显著增加（265.7 +/- 75.5 vs. 335.6 +/- 107.4 pmol/kg/天P < 0.05）。8-OHdG含量在训练结束后的第一天与训练前相比无显著性差异。血浆TBARS、LDH、CK、CK-MB和肌红蛋白在训练后显著升高（P < 0.05）。血浆β-胡萝卜素水平在夏令营后呈上升趋势，而血浆α-生育酚水平在夏令营后显著升高（P < 0.05）。这些结果表明，反复运动增强氧化应激和DNA也被运动诱导的活性氧损伤。然而，对DNA的氧化损伤并不是通过连续的运动积累的，尽管只要重复运动，它就会持续下去。
The purpose of this study was to investigate the effect of repeated exercise on oxidative damage to DNA in 10 well trained long distance runners who participated in an 8-day training camp. The average running distance during the camp was 30 +/- 3 km/day. The amount of urinary 8-hydroxy-deoxyguanosine (8-OHdC) excretion was used to estimate the oxidative DNA damage. Urine samples were collected for both a 3-day control period as well as throughout the camp. Blood samples were drawn after overnight fasting both before and after the camp. Urinary 8-OHdG excretion was significantly increased during the camp compared to the control period (265.7 +/- 75.5 vs. 335.6 +/- 107.4 pmol/kg/day P < 0.05). The content of 8-OHdG in the lymphocyte DNA on the day after finishing the camp did not differ from that before the camp. Plasma TBARS, LDH, CK, CK-MB, and myoglobin significantly rose after the camp (P < 0.05). The plasma beta-carotene levels tended to rise after the camp, while the plasma alpha-tocopherol levels increased significantly after the camp (P < 0.05). These results indicate that repeated exercise augments oxidative stress and that DNA is also injured by exercise-induced reactive oxygen species. However, the oxidative damage to DNA is not accumulated by consecutive exercise, although it is sustained as long as the exercise is repeated.