Nucleobase-involved native chemical ligation: a novel reaction between an oxanine nucleobase and N-terminal cysteine for oligonucleotide-peptide conjugation
Nucleobase-involved native chemical ligation: a novel reaction between an oxanine nucleobase and N-terminal cysteine for oligonucleotide-peptide conjugation
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DOI：
10.1039/c9cc08808c
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发表时间：
2020-05-21
影响因子：
4.9
通讯作者：
Pack，Seung Pil
中科院分区：
文献类型：
--
作者：
Jang，Eui Kyoung;Koike，Yohei;Pack，Seung Pil
在生物共轭化学中，实现未修饰氨基酸的靶标特异性反应具有挑战性。在这里，我们报告了一种新型核碱基涉及的天然化学连接（NbCL），它允许通过恶嗪核碱基和 N 末端半胱氨酸之间的新 S-N 酰基转移反应进行位点特异性寡核苷酸-肽缀合。
In bioconjugation chemistry, achieving a target-specific reaction for a non-modified amino acid is challenging. Here, we report a novel nucleobase-involved native chemical ligation (NbCL) that allows a site-specific oligonucleotide–peptide conjugation via a new S–N acyl transfer reaction between an oxanine nucleobase and N-terminal cysteine.