Acute inhibition of a cortical motor area impairs vocal control in singing zebra finches.
Acute inhibition of a cortical motor area impairs vocal control in singing zebra finches.
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皮质运动区的急性抑制会损害唱歌斑胸草雀的声音控制。
DOI：
10.1111/ejn.12757
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Lazarus,Michael
中科院分区：
文献类型：
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作者：
Yazaki-Sugiyama,Yoko;Yanagihara,Shin;Fuller,PatrickM;Lazarus,Michael
遗传靶向的方法，允许急性和可逆的操纵神经元回路活动，使一个前所未有的理解如何离散的神经元回路控制动物的行为。斑胸草雀的鸣叫行为已经成为研究行为运动序列产生和学习的神经元回路机制的一个很好的模型。我们采用了一种新开发的，可逆的，神经元沉默系统斑胸草雀测试的假设，即合奏的神经元的鲁棒核arcopallium（RA）控制特定的歌曲部分的声学结构，但不是时间，也不是歌曲元素的顺序。伊维菌素门控氯离子通道的亚基在RA神经元亚群中表达，配体给药始终抑制神经元兴奋性。一组RA神经元的活动受到抑制，导致鸟类用退化的元素唱歌，尽管歌曲元素的顺序不受影响。此外，有些音节在歌曲母题的中间或结尾消失。因此，我们的数据表明，特定的歌曲部分的生成是由RA神经元的一个子集控制的，而整个歌曲的元素顺序协调和时间控制的一个更高的前运动区。
Genetically targeted approaches that permit acute and reversible manipulation of neuronal circuit activity have enabled an unprecedented understanding of how discrete neuronal circuits control animal behavior. Zebra finch singing behavior has emerged as an excellent model for studying neuronal circuit mechanisms underlying the generation and learning of behavioral motor sequences. We employed a newly developed, reversible, neuronal silencing system in zebra finches to test the hypothesis that ensembles of neurons in the robust nucleus of the arcopallium (RA) control the acoustic structure of specific song parts, but not the timing nor the order of song elements. Subunits of an ivermectin‐gated chloride channel were expressed in a subset of RA neurons, and ligand administration consistently suppressed neuronal excitability. Suppression of activity in a group of RA neurons caused the birds to sing songs with degraded elements, although the order of song elements was unaffected. Furthermore some syllables disappeared in the middle or at the end of song motifs. Thus, our data suggest that generation of specific song parts is controlled by a subset of RA neurons, whereas elements order coordination and timing of whole songs are controlled by a higher premotor area.