Antitrypanosomal activity of some medicinal plants from Nigerian ethnomedicine
Antitrypanosomal activity of some medicinal plants from Nigerian ethnomedicine
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DOI：
10.1007/s00436-011-2516-z
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Oduola, Ayoade M.
中科院分区：
文献类型：
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作者：
Abiodun, Oyindamola O.;Gbotosho, Grace O.;Oduola, Ayoade M.
非洲人类锥虫病是一种被忽视的热带疾病，临床表现、诊断复杂，治疗困难。治疗锥虫病的现有药物陈旧、昂贵、疗效差、不良反应严重且面临耐药性问题。这种情况凸显了迫切需要开发新的、有效的、廉价的和安全的药物来治疗锥虫病。本研究中寻找新的抗锥虫药物是基于民族医学。针对来自尼日利亚民族药物的 10 种植物的 36 种植物提取物针对血流形式的布氏锥虫罗得西亚 STIB 900 的体外抗锥虫活性进行了评估。针对哺乳动物 L6 细胞测定了细胞毒性活性。阿尔马尔蓝测定用于测量抗锥虫和毒性测定的终点。罗勒叶的乙酸乙酯提取物。唇形科 (Labiatae) 显示出最高的抗锥虫活性（IC50 为 2.08 +/- 0.01 mu g/ml），选择性指数高达 29。此外，东方山黄精 (Trema orientalis (L.) Blume)（榆科）、Pericopsis laxiflora (Benth. ex Baker) Meeuwen 和麻疯树的己烷、乙酸乙酯或甲醇提取物。 （大戟科），榄仁树。 （Combretaceae）和 Vitex doniana Sweet（马鞭草科）表现出显着的抗锥虫活性（IC50 2.1-17.2 mu g/ml），对锥虫具有高选择性指数（20-80）。尼日利亚民族医学首次报道了 T. catappa 和 T. orientalis 对 T. brucei rhodesense (STIB 900) 的抗锥虫活性，这些植物可能是抗锥虫药物的潜在来源。
Human African trypanosomiasis is a neglected tropical disease with complex clinical presentation, diagnosis, and difficult treatment. The available drugs for the treatment of trypanosomiasis are old, expensive, and less effective, associated with severe adverse reactions and face the problem of drug resistance. This situation underlines the urgent need for the development of new, effective, cheap, and safe drugs for the treatment of trypanosomiasis. The search for new antitrypanosomal agents in this study is based on ethnomedicine. In vitro antitrypanosomal activity of 36 plant extracts from 10 plant species from Nigerian ethnomedicine was evaluated against bloodstream forms of Trypanosoma brucei rhodesiense STIB 900. Cytotoxic activity was determined against mammalian L6 cells. Alamar blue assay was used to measure the endpoint of both antitrypanosomal and toxicity assays. The ethyl acetate extract of leaves of Ocimum gratissimum Linn. (Labiatae) showed the highest antitrypanosomal activity (IC50 of 2.08 +/- 0.01 mu g/ml) and a high selective index of 29. Furthermore, the hexane, ethyl acetate, or methanol extracts of Trema orientalis (L.) Blume (Ulmaceae), Pericopsis laxiflora (Benth. ex Baker) Meeuwen, Jatropha curcas Linn. (Euphorbiaceae), Terminalia catappa Linn. (Combretaceae), and Vitex doniana Sweet (Verbenaceae) displayed remarkable antitrypanosomal activity (IC50 2.1-17.2 mu g/ml) with high selectivity indices (20-80) for trypanosomes. The antitrypanosomal activity of T. catappa and T. orientalis against T. brucei rhodesiense (STIB 900) is being reported for the first time in Nigerian ethnomedicine, and these plants could be a potential source of antitrypanosomal agents.