Complex associations between membrane proteins analyzed by analytical ultracentrifugation: Studies on the erythrocyte membrane proteins band 3 and ankyrin
Complex associations between membrane proteins analyzed by analytical ultracentrifugation: Studies on the erythrocyte membrane proteins band 3 and ankyrin
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通过分析超速离心分析膜蛋白之间的复杂关联：红细胞膜蛋白带 3 和锚蛋白的研究
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
D. Schubert
中科院分区：
文献类型：
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作者：
K. Mulzer;L. Kampmann;P. Petrasch;D. Schubert
不同水溶性蛋白之间的关联可以通过在分析式超离心机中进行沉降平衡实验和随后对所得的c(r)-分布进行数学分析来研究。分析可以通过用一种吸收波长为100 ~ 300纳米的染料标记其中一种蛋白质来简化。该方法也可应用于非离子洗涤剂溶液中的固有膜蛋白。本文综述了该方法并报道了其在人类红细胞膜两种蛋白质之间关联的应用：1)带3，膜的主要内在蛋白质，在洗涤溶液中，可能也在红细胞膜中，处于单体/二聚体/四聚体的结合平衡；2)细胞骨架蛋白锚定蛋白，通过结合带3将膜骨架与脂质双分子层连接起来。锚蛋白用异硫氰酸荧光素标记，使用的洗涤剂为C12E9。结果发现，大量出现的锚蛋白和带3的聚集体是一个锚蛋白分子和四个带3分子的复合物。这强烈提示，在红细胞膜中，带3四聚体代表高亲和力的锚蛋白结合位点。
Associations between different water-soluble proteins can be studied by sedimentation equilibrium experiments in the analytical ultracentrifuge and subsequent mathematical analysis of thec(r)-distributions obtained. The analysis can be simplified by labelling one of the proteins with a dye absorbing at wavelengths >300 nm. The method can also be applied to intrinsic membrane proteins in solutions of a nonionic detergent. The present paper both reviews the method and reports application to the associations between two proteins of the human erythrocyte membrane: 1) band 3, the membrane's main intrinsic protein which, in detergent solutions and presumably also in the erythrocyte membrane, is in a monomer/dimer/tetramer association equilibrium, and 2) the cytoskeletal protein ankyrin which links the membrane skeleton to the lipid bilayer by binding to band 3. Ankyrin was labelled with fluorescein isothiocyanate and the detergent used was C12E9. It was found that the only aggregate of ankyrin and band 3 occurring in significant amounts was a complex of one ankyrin molecule and four band 3 molecules. This strongly suggests that, in the erythrocyte membrane, the band 3 tetramer represents the high affinity ankyrin binding site.
锚蛋白与红细胞膜和脂囊泡中带 3 的重新结合。
DOI：
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发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Hargreaves,WR;Giedd,KN;Verkleij,A;Branton,D
通讯作者：
Branton,D
参与人红细胞膜-细胞骨架关联的蛋白质：血影蛋白、锚蛋白和带 3。
影响因子：
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作者：
Bennett,V
通讯作者：
Bennett,V