Two modes of survival of fission yeast without telomerase
Two modes of survival of fission yeast without telomerase
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DOI：
10.1126/science.282.5388.493
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发表时间：
1998-10-16
期刊：
影响因子：
56.9
通讯作者：
Cech, TR
中科院分区：
文献类型：
--
作者：
Nakamura, TM;Cooper, JP;Cech, TR
在裂糖菌中，端粒酶催化亚基基因trt1（+）的缺失导致大多数细胞死亡，但一个亚群存活。这里显示，大多数幸存者的所有染色体都循环化了，而一小部分人可能通过重组维持了他们的端粒。当端粒dna结合基因taz1（+）也被删除时，trt1(-) taz1（-）幸存者更频繁地使用重组模式。此外，taz1（-）细胞端粒的大量伸长在双突变体中不存在。因此，Taz1p似乎在裂变酵母中调节端粒重组和端粒酶活性。
Deletion of the telomerase catalytic subunit gene trt1(+) in Schizosaccharomyces pombe results in death for the majority of cells, but a subpopulation survives. Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller number maintain their telomeres presumably through recombination. When the telomeric DNA-binding gene taz1(+) is also deleted, trt1(-) taz1(-) survivors use the recombinational mode more frequently. Moreover, the massive elongation of telomeres in taz1(-) cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast.