Purification of fumonisin B2 isolated from rice culture
Purification of fumonisin B2 isolated from rice culture
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DOI：
10.1021/jf9608276
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发表时间：
1997-08
影响因子：
6.1
通讯作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
中科院分区：
文献类型：
--
作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
程序的生产，分离和纯化的真菌毒素伏马菌素B2从培养的串珠镰刀菌MRC 826生长在水稻。用乙腈：水提取伏马菌素B1、B2和B3，过滤提取物，并用旋转蒸发仪减少体积。通过制备型反相液相色谱法（LC）从其它伏马菌素B1和B3中分离出伏马菌素B2。将含有伏马菌素B2的级分浓缩以除去乙腈，并将剩余的水级分冷冻并冷冻干燥。将冷冻干燥的材料溶解在最少量的甲醇中，并在硅胶涂层板上通过制备型离心加速径向薄层色谱法进行分离。使用精确控制浸提物向吸附剂材料的施加速率的施加器来最小化浸提物带的宽度。用（A）氯仿：丙酮（4：3）和（B）氯仿：丙酮（4：3）的线性梯度从旋转板上洗脱级分。
Procedures are presented for the production, isolation, and purification of the mycotoxin fumonisin B2 from cultures of Fusarium moniliforme MRC 826 grown on rice. Fumonisins B1, B2, and B3 were extracted with acetonitrile:water, and the extracts were filtered and reduced in volume with a rotary evaporator. Fumonisin B2 was isolated from the other fumonisins B1 and B3 by preparative reversed-phase liquid chromatography (LC). Fractions that contained fumonisin B2 were concentrated to remove acetonitrile, and the remaining water fraction was frozen and freeze dried. The freeze-dried material was dissolved in a minimum amount of methanol and separated by preparative, centrifugally accelerated, radial, thin-layer chromatography on a silica gel-coated plate. An applicator that precisely controlled the rate of application of extract to the sorbent material was used to minimize the width of the band of extract. Fractions were eluted from the spinning plate with a linear gradient of (A) chloroform:acetone (4:3) a...