Isolation and characterisation of two MATE genes in rye
Isolation and characterisation of two MATE genes in rye
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DOI：
10.1071/fp09265
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Ma, Jian Feng
中科院分区：
文献类型：
--
作者：
Yokosho, Kengo;Yamaji, Naoki;Ma, Jian Feng
MATE蛋白广泛存在于细菌、真菌、植物和哺乳动物中。最近的研究表明，一组植物MATE基因编码柠檬酸转运蛋白，参与铝的解毒或铁从根向地上部的转运。在这项研究中，我们分离了两个同源基因（ScFRDL1和ScFRDL2）从这个家庭在黑麦（黑麦）。ScFRDL1与大麦（HordeumvulgareL.）ScFRDL2与水稻（Oryza sativa L.）中一个可能的铝响应蛋白OsFRDL2的同源性为80.6%。这两个基因主要在根中表达，但它们表现出不同的表达模式。ScFRDL1的表达不受Al处理的影响，但受Fe缺乏处理的上调。与此相反，ScFRDL2的表达，大大诱导铝，但不是由铁缺乏。铝诱导的ScFRDL2表达上调在根尖和基底根中均存在。此外，ScFRDL2的表达模式与柠檬酸分泌模式一致。免疫组化结果显示ScFRDL1定位于根尖、中央柱和基底根内皮层的所有细胞。两者合计，我们的研究结果表明，ScFRDL1参与流出的柠檬酸盐进入木质部的铁转运从根到芽，而ScFRDL2参与铝激活的柠檬酸盐分泌黑麦。
Multidrug and toxic compound extrusion (MATE) proteins are widely present in bacteria, fungi, plants and mammals. Recent studies have showed that a group of plant MATE genes encodes citrate transporter, which are involved in the detoxification of aluminium or translocation of iron from the roots to the shoots. In this study, we isolated two homologous genes (ScFRDL1 and ScFRDL2) from this family in rye (Secale cereale L.). ScFRDL1 shared 94.2% identity with HvAACT1, an Al-activated citrate transporter in barley (Hordeum vulgare L.) and ScFRDL2 shared 80.6% identity with OsFRDL2, a putative Al-responsive protein in rice (Oryza sativa L.). Both genes were mainly expressed in the roots, however, they showed different expression patterns. Expression of ScFRDL1 was unaffected by Al treatment, but up-regulated by Fe-deficiency treatment. In contrast, expression of ScFRDL2 was greatly induced by Al but not by Fe deficiency. The Al-induced up-regulation of ScFRDL2 was found in both the root tips and basal roots. Furthermore, the expression pattern of ScFRDL2 was consistent with citrate secretion pattern. Immunostaining showed that ScFRDL1 was localised at all cells in the root tips and central cylinder and endodermis in the basal root. Taken together, our results suggest that ScFRDL1 was involved in efflux of citrate into the xylem for Fe translocation from the roots to the shoots, while ScFRDL2 was involved in Al-activated citrate secretion in rye.