Osteogenic differentiation of human dental papilla mesenchymal cells
Osteogenic differentiation of human dental papilla mesenchymal cells
复制标题
DOI：
10.1016/j.bbrc.2006.02.101
复制
发表时间：
2006-04-21
影响因子：
3.1
通讯作者：
Ohgushi, H
中科院分区：
文献类型：
--
作者：
Ikeda, E;Hirose, M;Ohgushi, H
我们从阻生的人磨牙中分离牙乳头，并在胶原酶处理牙乳头后增殖贴壁成纤维细胞。这些细胞的造血标志物呈阴性，但CD 29、CD 44、CD 90、CD 105和CD 166呈阳性。当细胞在β-甘油磷酸盐、抗坏血酸和地塞米松存在下进一步培养14天时，观察到矿化区域以及由高碱性磷酸酶活性和骨钙素含量证明的成骨分化。在蛋白质和基因表达水平上证实了分化。细胞也可以冷冻保存，解冻后可以显示体内骨形成能力。这些结果表明，间充质型细胞定位于牙乳头和细胞可以培养扩增/用于骨组织工程。(c)2006年爱思唯尔公司All rights reserved.
We isolated dental papilla from impacted human molar and proliferated adherent fibroblastic cells after collagenase treatment of the papilla. The cells were negative for hematopoietic markers but positive for CD29, CD44, CD90, CD105, and CD166. When the cells were further cultured in the presence of beta-glycerophosphate, ascorbic acid, and dexamethasone for 14 days, mineralized areas together with osteogenic differentiation evidenced by high alkaline phosphatase activity and osteocalcin contents were observed. The differentiation was confirmed at both protein and gene expression levels. The cells can also be cryopreserved and, after thawing, could show in vivo bone-forming capability. These results indicate that mesenchymal type cells localize in dental papilla and that the cells can be culture expanded/utilized for bone tissue engineering. (c) 2006 Elsevier Inc. All rights reserved.