Development of an ultrasound microscope combined with optical microscope for multiparametric characterization of a single cell.
Development of an ultrasound microscope combined with optical microscope for multiparametric characterization of a single cell.
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开发超声波显微镜与光学显微镜相结合，用于单细胞的多参数表征。
DOI：
10.1109/tuffc.2014.006865
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Saijo Y
中科院分区：
文献类型：
--
作者：
Arakawa M;Shikama J;Yoshida K;Nagaoka R;Kobayashi K;Saijo Y
细胞的生物力学特性影响着动脉粥样硬化和肿瘤的病理状态，因而受到广泛关注。在本研究中，超声显微镜系统结合光学显微镜的表征与多个超声参数的单个细胞的发展。探头中心频率为375 MHz，扫描面积为80 × 80 μm，采样点最多可达200 × 200个。倒置光学显微镜被纳入该系统的设计，允许同时光学观察培养的细胞。多个超声参数的二维映射，例如声速、衰减和声阻抗，以及所研究的样本/细胞的厚度、密度和体积模量等，是由系统实现的。该系统成功地获得了3 T3-L1成纤维细胞的声速和厚度。超声显微镜系统结合光学显微镜进一步增强了我们对细胞生物力学的理解。
Biomechanics of the cell has been gathering much attention because it affects the pathological status in atherosclerosis and cancer. In the present study, an ultrasound microscope system combined with optical microscope for characterization of a single cell with multiple ultrasound parameters was developed. The central frequency of the transducer was 375 MHz and the scan area was 80 × 80 μm with up to 200 × 200 sampling points. An inverted optical microscope was incorporated in the design of the system, allowing for simultaneous optical observations of cultured cells. Two-dimensional mapping of multiple ultrasound parameters, such as sound speed, attenuation, and acoustic impedance, as well as the thickness, density, and bulk modulus of specimen/cell under investigation, etc., was realized by the system. Sound speed and thickness of a 3T3-L1 fibroblast cell were successfully obtained by the system. The ultrasound microscope system combined with optical microscope further enhances our understanding of cellular biomechanics.