Analyzing grooming microstructure in neurobehavioral experiments
Analyzing grooming microstructure in neurobehavioral experiments
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DOI：
10.1038/nprot.2007.367
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Tuohimaa, Pentti
中科院分区：
文献类型：
--
作者：
Kalueff, Allan V.;Aldridge, J. Wayne;Tuohimaa, Pentti
梳理毛发是啮齿类动物中一种常见且强烈的行为。它已被证明对许多实验因素高度敏感，使其成为理想的操纵目标。啮齿类动物梳理毛发的复杂模式通常沿头尾方向进行，并涉及几个不同的阶段，可以将其分解为其组成部分和微观结构。协议中描述了几种梳理图案分析方法，允许根据梳理活动及其排序的测量来评估这种行为。此外，可以根据修饰发生的区域分布和语法来评估修饰。由于具有强烈梳理表型的啮齿动物模型数量不断增加，这种高通量的深入分析对于生物医学研究变得至关重要。
Grooming is a commonplace, robust behavior in rodent species. It has been shown to be highly sensitive to a number of experimental factors, making it an ideal target for manipulation. The complex patterning of grooming in rodents, which usually proceeds in a cephalo-caudal direction and involves several distinct stages, can be dissected into its constituent parts and microstructures. Several grooming patterning analysis methods are described in the protocol that allow for an assessment of this behavior based on measurements of grooming activity and its sequencing. Additionally, grooming can be evaluated in reference to the regional distribution and syntax in which it occurs. Owing to the ever-increasing number of rodent models that have strong grooming phenotypes, this high-throughput in-depth analysis is becoming crucial for biomedical research.