Immunofluorescence Detection of Callose in Plant Tissue Sections.
Immunofluorescence Detection of Callose in Plant Tissue Sections.
复制标题
植物组织切片中胼胝质的免疫荧光检测。
DOI：
10.1007/978-1-0716-2132-5_10
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Amsbury S
中科院分区：
文献类型：
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作者：
Amsbury S
胞间连丝(PD)细胞壁组分的积累对植物共生体细胞间运输的调控至关重要。在这里，我们描述了使用单抗对切开的植物组织中的愈伤组织进行荧光成像的方案。该协议通过固定、嵌入和切片植物材料来暴露内细胞壁，从而获得高分辨率图像。通过将该方法与高分辨率共聚焦显微镜相结合，我们可以在各种植物组织和物种中检测到帕金森综合症。
The accumulation of the cell wall component callose at plasmodesmata (PD) is crucial for the regulation of symplastic intercellular transport in plants. Here we describe protocols to fluorescently image callose in sectioned plant tissue using monoclonal antibodies. This protocol achieves high-resolution images by the fixation, embedding, and sectioning of plant material to expose internal cell walls. By using this protocol in combination with high-resolution confocal microscopy, we can detect PD callose in a variety of plant tissues and species.