Comparison of cellular responses of mesenchymal stem cells derived from bone marrow and synovium on combined silk scaffolds
Comparison of cellular responses of mesenchymal stem cells derived from bone marrow and synovium on combined silk scaffolds
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骨髓和滑膜来源的间充质干细胞在组合丝支架上的细胞反应比较
DOI：
10.1002/jbm.a.35154
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Liu, Haifeng;Wei, Xing;Fan, Yubo
滑膜来源的间充质干细胞(SMSCs)作为间充质干细胞(MSCs)家族的新成员，越来越被认为是一种很有前途的肌肉骨骼再生治疗细胞。然而，很少有报道提到SMSCs的韧带形成，尤其是对其用于韧带再生的工程应用方面的报道很少。本研究的目的是研究和比较骨髓间充质干细胞和滑膜间充质干细胞在复合丝质支架上的细胞反应，以确定最适合组织工程韧带的细胞来源。将兔SMSCs和骨髓间充质干细胞(BMSCs)接种于复合丝素支架上，体外培养2周。对样本的细胞形态、增殖、胶原产生、韧带相关细胞外基质(ECM)标志物的基因和蛋白表达进行研究和比较。此外，将绿色荧光蛋白导入这两种细胞，以评估它们在膝关节内环境中种植后的命运。培养14d后，与BMSCs相比，SMSCs的增殖能力明显增强。SMSCs中韧带相关ECM标志物的转录和蛋白表达水平均显著高于BMSCs。此外，术后6周，SMSC负载的构建物比BMSC负载的构建物有更多的存活细胞。因此，从体内外的细胞反应来看，SMSCs可能比BMSCs更适合作为组织工程韧带进一步研究和开发的细胞来源。(C)2014 Wiley期刊，Inc.生物材料资源A部分：103A：115-125,2015。
As a brand new member in mesenchymal stem cells (MSCs) families, synovium-derived mesenchymal stem cells (SMSCs) have been increasingly regarded as a promising therapeutic cell species for musculoskeletal regeneration. However, there are few reports mentioning ligamentogenesis of SMSCs and especially null for their engineering use towards ligament regeneration. The aim of this study was to investigate and compare the cellular responses of MSCs derived from bone marrow and synovium on combined silk scaffolds that can be used to determine the cell source most appropriate for tissue-engineered ligament. Rabbit SMSCs and bone marrow-derived mesenchymal stem cells (BMSCs) were isolated and cultured in vitro for two weeks after seeding on the combined silk scaffolds. Samples were studied and compared for their cellular morphology, proliferation, collagen production, gene, and protein expression of ligament-related extracellular matrix (ECM) markers. In addition, the two cell types were transfected with green fluorescent protein to evaluate their fate after implantation in an intraarticular environment of the knee joint. After 14 days of culturing, SMSCs showed a significant increase in proliferation as compared with BMSCs. The transcript and protein expression levels of ligament-related ECM markers in SMSCs were significantly higher than those in BMSCs. Moreover, 6 weeks postoperatively, more viable cells were presented in SMSC-loaded constructs than in BMSC-loaded constructs. Therefore, based on the cellular response in vitro and in vivo, SMSCs may represent a more suitable cell source than BMSCs for further study and development of tissue-engineered ligament. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 115-125, 2015.