Fermented rice bran supplementation attenuates chronic colitis-associated extraintestinal manifestations in female C57BL/6N mice
Fermented rice bran supplementation attenuates chronic colitis-associated extraintestinal manifestations in female C57BL/6N mice
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DOI：
10.1016/j.jnutbio.2021.108855
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发表时间：
2021-10-06
影响因子：
5.6
通讯作者：
Shirakawa, Hitoshi
中科院分区：
文献类型：
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作者：
Islam, Jahidul;Agista, Afifah Zahra;Shirakawa, Hitoshi
炎症性肠病（IBD）患者的肠外表现（EIM）发生率较高，包括肝脏疾病、肌肉减少症和神经炎症。发酵米糠（FRB）是以米糠（RB）为原料，富含多种生物活性物质，具有抗结肠炎活性。然而，其在EIM预防中的作用仍不清楚。在这里，我们第一次研究了雌性C57 B1/6 N小鼠中的EIM是否通过FRB补充而减弱。用1.5%葡聚糖硫酸钠（DSS）饮水（4d）和饮水（12 d）重复给药诱导EIM。将小鼠分为3组-对照组（AIN 93 M）、10%RB组和10%FRB组。与RB和对照组相比，FRB通过显着降低血清中的促炎细胞因子Tnf-alpha和Il-6以及血清和肌肉中的晚期糖基化终产物特异性受体（Ager）来改善复发性结肠炎和肌肉炎症。由于FRB降低天冬氨酸转氨酶水平和氧化应激，它可能会预防肝脏疾病。FRB下调促炎细胞因子和趋化因子转录负责神经炎症在海马和上调G蛋白偶联受体（GPRs），Gpr 41和Gpr 43，在小肠和大肠，这可能解释FRB介导的保护机制。因此，FRB可作为预防IBD相关EIM的补充剂。(C)2021爱思唯尔公司All rights reserved.
Patients with inflammatory bowel disease (IBD) have higher incidence of extraintestinal manifestations (EIM), including liver disorders, sarcopenia, and neuroinflammation. Fermented rice bran (FRB), generated from rice bran (RB), is rich in bioactive compounds, and exhibits anti-colitis activity. However, its role in EIM prevention is still unclear. Here, for the first time, we investigated whether EIM in female C57Bl/6N mice is attenuated by FRB supplementation. EIM was induced by repeated administration of 1.5% dextran sulfate sodium (DSS) in drinking water (4 d) followed by drinking water (12 d). Mice were divided into 3 groups-control (AIN93M), 10% RB, and 10% FRB. FRB ameliorated relapsing colitis and inflammation in muscle by significantly lowering proinflammatory cytokines Tnf-alpha and Il-6 in serum and advanced glycation end product-specific receptor (Ager) in serum and muscle when compared with the RB and control groups. As FRB reduced aspartate aminotransferase levels and oxidative stress, it might prevent liver disorders. FRB downregulated proinflammatory cytokine and chemokine transcripts responsible for neuroinflammation in the hippocampus and upregulated mRNA expression of G protein coupled receptors (GPRs), Gpr41 and Gpr43, in small and large intestines, which may explain the FRB-mediated protective mechanism. Hence, FRB can be used as a supplement to prevent IBD-associated EIM. (C) 2021 Elsevier Inc. All rights reserved.