THE SMALL NONSTRUCTURAL PROTEIN-NS2 OF THE AUTONOMOUS PARVOVIRUS MINUTE VIRUS OF MICE IS REQUIRED FOR VIRUS GROWTH IN MURINE CELLS
THE SMALL NONSTRUCTURAL PROTEIN-NS2 OF THE AUTONOMOUS PARVOVIRUS MINUTE VIRUS OF MICE IS REQUIRED FOR VIRUS GROWTH IN MURINE CELLS
复制标题
DOI：
10.1099/0022-1317-73-7-1839
复制
发表时间：
1992-07-01
影响因子：
3.8
通讯作者：
PINTEL, DJ
中科院分区：
文献类型：
--
作者：
CATER, JE;PINTEL, DJ
小鼠自主细小病毒（MVM）株MVM（p）和MVM（i）的突变体不能产生或产生截短的NS2蛋白，在感染一系列正常和转化的鼠细胞系后，在感染性病毒的产生方面存在缺陷，并且获得比野生型病毒更低水平的病毒DNA合成。突变病毒的生长和DNA复制水平与测试的大鼠、仓鼠和人类品系中的野生型病毒相似。这些结果表明，NS2对MVM生长的需求是鼠种特异性的。
Mutants of the autonomous parvovirus minute virus of mice (MVM) strains MVM(p) and MVM(i) that either fail to produce or produce a truncated NS2 protein, were deficient in the production of infectious virus and attained lower levels of viral DNA synthesis than wild-type virus following infection of a series of normal and transformed murine cell lines. Mutant virus growth and the levels of DNA replication were similar to those of wild-type virus in the rat, hamster and human lines tested. These results suggest that the requirement of NS2 for the growth of MVM is murine species-specific.