SHIFTS IN INTERLEUKIN-4 AND INTERFERON-GAMMA PRODUCTION BY T-CELLS OF PATIENTS WITH ELEVATED SERUM IGE LEVELS AND THE MODULATORY EFFECTS OF THESE LYMPHOKINES ON SPONTANEOUS IGE SYNTHESIS
SHIFTS IN INTERLEUKIN-4 AND INTERFERON-GAMMA PRODUCTION BY T-CELLS OF PATIENTS WITH ELEVATED SERUM IGE LEVELS AND THE MODULATORY EFFECTS OF THESE LYMPHOKINES ON SPONTANEOUS IGE SYNTHESIS
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DOI：
10.1016/0091-6749(91)90213-8
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发表时间：
1991-01-01
影响因子：
14.2
通讯作者：
DEVRIES, JE
中科院分区：
文献类型：
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作者：
ROUSSET, F;ROBERT, J;DEVRIES, JE
白细胞介素-4（IL-4）已被证明可诱导健康供体外周血单个核细胞（PBMNCs）合成IgE。 在这项研究中，我们证明了IL-4也可以增强过敏性/特应性患者和Buckley综合征或高IgE综合征患者的PBMNC的自发IgE合成。 这些患者的PBMNC自发IgE产生被干扰素（IFN）-γ或IFN-α以剂量依赖性方式抑制。 尽管血清IgE水平高，但在患者血清中未检测到IL-4，但获得的间接证据表明，体内IL-4产生的增强可能与这些患者的高血清IgE水平有关。 在4/21名受试患者的PBMNC培养物中测量自发IL-4产生。 此外，自发IgE合成的PBMNCs的其他三名患者在体外测试的抗IL-4抗血清部分阻断。 此外，患者血清中可溶性CD 23（由IL-4特异性诱导）的水平显著升高。 最后，患者PBMC的活化导致IL-4和IFN-γ合成的水平分别高于和低于平行测试的健康对照供体的PBMC产生的水平。 总的来说，我们的数据表明，自发性IgE合成在体外可以通过IL-4，IL-5，IFN-γ，和IFN-α调节。 此外，我们的数据表明，增强IL-4和减少IFN-γ的产生与这些患者中观察到的血清IgE水平升高有关。
Interleukin-4 (IL-4) has been demonstrated to induce IgE synthesis by peripheral blood mononuclear cells (PBMNCs) of healthy donors. In this study, we demonstrated that IL-4 also can enhance spontaneous IgE synthesis by PBMNCs of allergic/atopic patients and patients with Buckley's or hyper-IgE syndrome. Spontaneous IgE production by PBMNCs of these patients was suppressed by interferon (IFN)-gamma or IFN-alpha in a dose-dependent fashion. Despite high serum IgE levels, no IL-4 could be detected in the serum of the patients, but indirect evidence was obtained indicating that enhanced IL-4 production in vivo may be associated with the high serum-IgE levels in these patients. Spontaneous IL-4 production was measured in PBMNC cultures of 4/21 patients tested. Furthermore, spontaneous IgE synthesis by PBMNCs of three other patients of seven tested in vitro was partly blocked by anti-IL-4 antiserum. In addition, levels of soluble CD23 (which is specifically induced by IL-4) were strongly elevated in sera of patients. Finally, activation of PBMNCs of the patients resulted in levels of IL-4 and of IFN-gamma synthesis that were higher and lower, respectively, than levels produced by PBMNCs of healthy control donors tested in parallel. Collectively, our data indicate that spontaneous IgE synthesis in vitro can be modulated by IL-4, IL-5, IFN-gamma, and IFN-alpha. In addition, our data suggest that enhanced IL-4 and reduced IFN-gamma production is associated with the elevated serum IgE levels observed in these patients.